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Scientific article
English

Fluorometric Detection of Enzyme Activity with Synthetic Supramolecular Pores

Published inScience, vol. 298, no. 5598, p. 1600-1602
Publication date2002
Abstract

The reversible blockage of synthetic pores formed by rigid-rod beta barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with "naked-eye" fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod beta barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis.

Citation (ISO format)
DAS, Gopal, TALUKDAR, Pinaki, MATILE, Stefan. Fluorometric Detection of Enzyme Activity with Synthetic Supramolecular Pores. In: Science, 2002, vol. 298, n° 5598, p. 1600–1602. doi: 10.1126/science.1077353
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ISSN of the journal0036-8075
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