Scientific article

Topological Diversity of Artificial ß-Barrels in Water

Published inChirality, vol. 13, no. 3, p. 170-176
Publication date2001

Rigid-rod ß-barrels are composed of interdigitating, short, amphiphilic peptide strands flanked by stabilizing rigid-rod staves. We here report studies on the topological diversity of these recently devised artificial -barrels with regard to their length. For this purpose, homologous p-octiphenyl, p-sexiphenyl, and p-quarterphenyl rods were equipped with complementary tripeptide strands based on the sequences Lys-Leu-Lys and Glu-Leu-Glu. The stability of rigid-rod -barrels of different length was determined by denaturation with guanidinium chloride. Free energies of δGH2O = -5.2 kcalmol-1, δGH2O = -2.9 kcalmol-1, and δGH2O < -0.3 kcalmol-1 found for homologous p-octiphenyl, p-sexiphenyl, and p-quarterphenyl -barrels demonstrated strong dependence of -barrel stability on ß-barrel length. These results revealed a very qualitative minimal (~23 Å) and an ideal -barrel length (~34 Å), synergistic formation (α = 1.4) and remarkable stability for ideal p-octiphenyl -barrels exceeding that of several proteins and most synthetic models. Rigid-rod ß-barrels with p-oligophenyl staves longer than 34 Å will be very difficult to make and study because of rapidly decreasing rod solubilities. However, a strategy to bypass this apparent upper limitation of -barrel length is introduced: supramolecular matching of mismatched rods yielded elongated -ß-barrels (61 Å) of acceptable stability (δGH2O = 2.2 - 3.1 kcalmol-1).

Citation (ISO format)
DAS, Gopal, MATILE, Stefan. Topological Diversity of Artificial ß-Barrels in Water. In: Chirality, 2001, vol. 13, n° 3, p. 170–176. doi: 10.1002/1520-636X(2001)13:3<170::AID-CHIR1016>3.0.CO;2-6
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ISSN of the journal0899-0042

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