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Gentamicin inactivation in purulent exudates: role of cell lysis

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Published in The journal of infectious diseases. 1980, vol. 142, no. 4, p. 586-593
Abstract Factors contributing to the binding and reversible inactivation of gentamicin by purulent exudates were studied in a simplified in vitro model consisting of purified human polymorphonuclear leukocytes (PMNLs). Whereas intact PMNLs (10(6)-10(8)/ml) bound almost no [14C]gentamicin, freeze-thawed PMNLs showed extensive [14C]gentamicin binding, expressed as antibiotic cosedimenting with particulate material from the lysed PMNLs. Antibiotic binding could be related to the concentration of lysed PMNLs and to the amount of [14C]gentamicin added. Binding of [14C]gentamicin by lysed PMNLs was highly sensitive to DNase I but was unaffected by RNase, Triton X-100, or protease. Purified chromatin or DNA from either purulent exudates or lysed PMNLs reproduced the [14C]gentamicin-binding pattern obtained with crude PMNL lysate. These results show that gentamicin inactivation in purulent exudates can be correlated with binding of the antibiotic to lysed PMNLs; PMNL chromatin DNA is identified as one of the major binding factors.
Keywords Aminoglycosides/antagonists & inhibitorsBinding SitesCell SurvivalDnaDrug Resistance, MicrobialExudates and Transudates/ microbiologyGentamicins/ antagonists & inhibitorsHumansNeutrophils
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PMID: 7441018
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VAUDAUX, Pierre, WALDVOGEL, Francis. Gentamicin inactivation in purulent exudates: role of cell lysis. In: The Journal of infectious diseases, 1980, vol. 142, n° 4, p. 586-593. doi: 10.1093/infdis/142.4.586 https://archive-ouverte.unige.ch/unige:7664

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Deposited on : 2010-06-21

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