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Robust G2 pausing of adult stem cells in Hydra

Crescenzi, Marco
Published in World Biomedical Frontiers. 2014, vol. 87, no. 1-2, p. 83-99
Abstract The importance of this study is at two levels, first it provides a fast and reliable procedure to analyze the cycling profile of small amounts of tissues obtained in multiple contexts. Second, it provides a framework to study the impact of G2 pausing on regenerative processes. Indeed Hydra adult stem cells share similarities with the mammalian embryonic stem cells, characterized by a lack of G1 phase and an extended G2 phase. Cyclin-E is overexpressed in these cells, supporting the fact that stem cells do not stop at the G1/S checkpoint. A correlation between G2 pausing and regeneration is not only observed in Hydra, but also in Axolotl, in mice. Therefore it is important to understand how G2 pausing might positively impact the initiation of tissue repair and regeneration. We propose four types of “pro-regenerative” benefits linked to an extended G2 phase (Figure 2): (1) an extended G2 phase might enhance the DNA repair program, as homologous recombination, one of the well conserved mechanisms involved in the repair of double strand DNA is activated during late S-phase and G2, (2) G2 paused cells show an enhanced resistance to cell death, possibly linked to G2 check-point proteins as observed in mammalian epithelial stem cells [5], (3) G2 pausing favors an immediate entry into mitosis upon injury as shown by Hydra ISCs, (4) in the framework of a remodeling process that characterizes Hydra regeneration, epithelial cells can directly differentiate in G2 in response to injury.
Keywords Adult stem cellsCell cycleRegenerationHydraG2 pausing
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Research group Groupe Galliot
Projects FNS: Grants 31003A_130337, 31003A_149630
Claraz Donation
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BUZGARIU, Wanda Christa, CRESCENZI, Marco, GALLIOT, Brigitte. Robust G2 pausing of adult stem cells in Hydra. In: World Biomedical Frontiers, 2014, vol. 87, n° 1-2, p. 83-99. https://archive-ouverte.unige.ch/unige:73076

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Deposited on : 2015-06-09

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