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Titles listAnalysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR
Titles listAnalysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR
Scientific Article
unige:7171 
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Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR |
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| Published in | FEMS microbiology letters. 2006, vol. 254, no. 2, p. 217-225 | |
| Abstract | In Pseudomonas aeruginosa many of the clinically relevant resistance mechanisms result from changes in gene expression as exemplified by the Mex drug efflux pumps, the AmpC beta-lactamase and the carbapenem-specific porin OprD. We used quantitative real-time-PCR to analyze the expression of these genes in susceptible and antibiotic-resistant laboratory and clinical strains. In nalB mutants, which overexpress OprM, we observed a four- to eightfold increase in the expression of mexA, mexB, and oprM genes. MexX and mexY genes were induced eight to 12 times in the presence of 2 mg L(-1) tetracycline. The mexC/oprJ and mexE/oprN gene expression levels were increased 30- to 250-fold and 100- to 760-fold in nfxB and nfxC mutants, respectively. We further found that in defined laboratory strains expression levels of ampC and oprD genes paralleled beta-lactamase activity and OprD protein levels, respectively. Our data support the use of quantitative real-time-PCR chain reaction for the analysis of the antimicrobial resistance gene expression in P. aeruginosa. | |
| Keywords | Anti-Bacterial Agents/ pharmacology — Bacterial Proteins/genetics/ metabolism — Drug Resistance, Bacterial/ genetics — Gene Expression Regulation, Bacterial — Humans — Porins/genetics/metabolism — Pseudomonas aeruginosa/ drug effects/genetics/metabolism — Reverse Transcriptase Polymerase Chain Reaction/ methods — Beta-Lactamases/genetics/metabolism | |
| Identifiers | PMID: 16445748 | |
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| Citation (ISO format) | DUMAS, Jean-Luc Claude et al. Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR. In: FEMS microbiology letters, 2006, vol. 254, n° 2, p. 217-225. doi: 10.1111/j.1574-6968.2005.00008.x https://archive-ouverte.unige.ch/unige:7171 |
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