Scientific article

Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes

Published inGenes & development, vol. 23, no. 8, p. 939-950
Publication date2009

Transgenerational epigenetic inheritance has been defined by the study of relatively few loci. We examined a population of recombinant inbred lines with epigenetically mosaic chromosomes consisting of wild-type and CG methylation-depleted segments (epiRILs). Surprisingly, transposons that were immobile in the parental lines displayed stochastic movement in 28% of the epiRILs. Although analysis after eight generations of inbreeding, supported by genome-wide DNA methylation profiling, identified recombined parental chromosomal segments, these were interspersed with unexpectedly high frequencies of nonparental methylation polymorphism. Hence, epigenetic inheritance in hybrids derived from parents with divergent epigenomes permits long-lasting epi-allelic interactions that violate Mendelian expectations. Such persistently "unstable" epigenetic states complicate linkage-based epigenomic mapping. Thus, future epigenomic analyses should consider possible genetic instabilities and alternative mapping strategies.

  • Arabidopsis/genetics/metabolism
  • Arabidopsis Proteins/genetics
  • Chromosome Mapping
  • Chromosomes, Plant/genetics
  • DNA (Cytosine-5-)-Methyltransferase/genetics
  • DNA Methylation/genetics
  • DNA Transposable Elements/genetics
  • Epigenesis, Genetic/genetics
  • Genes, Plant/genetics
  • Genome, Plant/genetics
  • Genomic Instability/genetics
  • Inbreeding
  • Mosaicism
  • Phenotype
  • Recombination, Genetic
Citation (ISO format)
REINDERS, Jon et al. Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes. In: Genes & development, 2009, vol. 23, n° 8, p. 939–950. doi: 10.1101/gad.524609
Main files (1)
Article (Published version)
ISSN of the journal1549-5477

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