Simplification of a chromatographic test methodology for evaluation of base deactivated supports

Stella, Cinzia; Seuret, Patrick; Rudaz, Serge; Tchapla, A.; Gauvrit, J. -Y.; Lanteri, P.; Veuthey, Jean-Luc

doi:10.1007/BF02492466

Scientific article

English

Simplification of a chromatographic test methodology for evaluation of base deactivated supports

Contributors

Stella, Cinzia; Seuret, Patrick; Rudaz, Serge

; Tchapla, A.; Gauvrit, J. -Y.; Lanteri, P.; Veuthey, Jean-Luc

Published in

Chromatographia, vol. 56, no. 11-12, p. 665-671

Publication date

2002

Abstract

Today reversed-phase liquid chromatography (RP-LC) is the most employed chromatographic technique for analysis of basic compounds. Unfortunately, the strong ionic interactions of basic compounds with residual silanols result in asymmetric peaks and non-reproducible retention. This problem and the continous increase in the use of RP-LC techniques have furthered the need for a new generation of “base deactivated” stationary phases. Selecting the appropriate stationary phase for a specific separation is an important parameter in the development of LC methods. To characterize and evaluate the relative chromatographic performance of stationary phases, a series of tests are proposed in the literature. In this work, a chromatographic test previously developed is discussed with the aim of simplifying its methodology. The number of test compounds was reduced (seven instead of fourteen), as well as that of the mobile phases (one or two instead of three) and columns per support (three instead of five). This simplification allows a faster and easier method to evaluate new chromatographic supports.

Keywords

Column liquid chromatography
Principal component analysis
Chromatographic test method
Basic compounds

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

STELLA, Cinzia et al. Simplification of a chromatographic test methodology for evaluation of base deactivated supports. In: Chromatographia, 2002, vol. 56, n° 11-12, p. 665–671. doi: 10.1007/BF02492466

Article (Published version)

Identifiers

PID : unige:6782
DOI : 10.1007/BF02492466

Additional URL for this publication

http://www.springerlink.com/index/10.1007/BF02492466

Journal ISSN

0009-5893

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Creation

04/06/2010 12:00:00

First validation

04/06/2010 12:00:00

Update

26/03/2026 10:49:13

Status update

26/03/2026 10:49:13

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26/03/2026 10:55:53

Simplification of a chromatographic test methodology for evaluation of base deactivated supports

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation