Scientific article
Open access

LTP promotes a selective long-term stabilization and clustering of dendritic spines

Published inPLoS biology, vol. 6, no. 9, e219
Publication date2008

Dendritic spines are the main postsynaptic site of excitatory contacts between neurons in the central nervous system. On cortical neurons, spines undergo a continuous turnover regulated by development and sensory activity. However, the functional implications of this synaptic remodeling for network properties remain currently unknown. Using repetitive confocal imaging on hippocampal organotypic cultures, we find that learning-related patterns of activity that induce long-term potentiation act as a selection mechanism for the stabilization and localization of spines. Through a lasting N-methyl-D-aspartate receptor and protein synthesis-dependent increase in protrusion growth and turnover, induction of plasticity promotes a pruning and replacement of nonactivated spines by new ones together with a selective stabilization of activated synapses. Furthermore, most newly formed spines preferentially grow in close proximity to activated synapses and become functional within 24 h, leading to a clustering of functional synapses. Our results indicate that synaptic remodeling associated with induction of long-term potentiation favors the selection of inputs showing spatiotemporal interactions on a given neuron.

  • Dendritic Spines
  • Electrophysiology
  • Excitatory Postsynaptic Potentials
  • Hippocampus
  • Long-Term Potentiation
  • Neuronal Plasticity
  • Neurons
  • Pyramidal Cells
  • Rats
  • Synapses
  • Synaptic Transmission
  • Tissue Culture Techniques
Citation (ISO format)
DE ROO, Mathias, KLAUSER, Paul, MULLER, Dominique. LTP promotes a selective long-term stabilization and clustering of dendritic spines. In: PLoS biology, 2008, vol. 6, n° 9, p. e219. doi: 10.1371/journal.pbio.0060219
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Article (Published version)
ISSN of the journal1544-9173

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Creation11/26/2008 3:15:00 PM
First validation11/26/2008 3:15:00 PM
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