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Dysfunction of mitochondria Ca2+ uptake in cystic fibrosis airway epithelial cells

Raveau, Dorothée
Becq, Frédéric
Vandebrouck, Clarisse
Published in Mitochondrion. 2009, vol. 9, no. 4, p. 232-41
Abstract In the genetic disease cystic fibrosis (CF), the most common mutation F508del promotes the endoplasmic reticulum (ER) retention of misfolded CF proteins. Furthermore, in homozygous F508del-CFTR airway epithelial cells, the histamine Ca(2+) mobilization is abnormally increased. Because the uptake of Ca(2+) by mitochondria during Ca(2+) influx or Ca(2+) release from ER stores may be crucial for maintaining a normal Ca(2+) homeostasis, we compared the mitochondria morphology and distribution by transmission electron microscopy technique and the mitochondria membrane potential variation (DeltaPsi(mit)) using a fluorescent probe (TMRE) on human CF (CF-KM4) and non-CF (MM39) tracheal serous gland cell lines. Confocal imaging of Rhod-2-AM-loaded or of the mitochondrial targeted cameleon 4mtD3cpv-transfected human CF and non-CF cells, were used to examine the ability of mitochondria to sequester intracellular Ca(2+). The present study reveals that (i) the mitochondria network is fragmented in F508del-CFTR cells, (ii) the DeltaPsi(mit) of CF mitochondria is depolarized compared non-CF mitochondria, and (iii) the CF mitochondria Ca(2+) uptake is reduced compared non-CF cells. We propose that these defects in airway epithelial F508del-CFTR cells are the consequence of mitochondrial membrane depolarization leading to a deficient mitochondrial Ca(2+) uptake.
Keywords Calcium/metabolismCell LineCystic Fibrosis/pathologyEpithelial Cells/metabolism/physiology/ultrastructureHumansMembrane Potential, MitochondrialMitochondria/metabolism/physiology/ultrastructureRespiratory System/pathology
PMID: 19249391
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Research groups Canaux ioniques endothéliaux (828)
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ANTIGNY, Fabrice et al. Dysfunction of mitochondria Ca2+ uptake in cystic fibrosis airway epithelial cells. In: Mitochondrion, 2009, vol. 9, n° 4, p. 232-41. doi: 10.1016/j.mito.2009.02.003 https://archive-ouverte.unige.ch/unige:5530

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Deposited on : 2010-03-23

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