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Title

NADPH oxidase 1 deficiency alters caveolin phosphorylation and angiotensin II-receptor localization in vascular smooth muscle
Authors
Basset, Olivier
Deffert-Delbouille, Christine
Foti, Michelangelo
Bedard, Karen
Jaquet, Vincent
Ogier-Denis, Eric
Krause, Karl-Heinz
Published in Antioxidants and Redox Signaling. 2009, vol. 11, no. 10, p. 2371-84
Abstract The superoxide-generating NADPH oxidase NOX1 is thought to be involved in signaling by the angiotensin II-receptor AT1R. However, underlying signaling steps are poorly understood. In this study, we investigated the effect of AngII on aortic smooth muscle from wild-type and NOX1-deficient mice. NOX1-deficient cells showed decreased basal ROS generation and did not produce ROS in response to AngII. Unexpectedly, AngII-dependent Ca(2+) signaling was markedly decreased in NOX1-deficient cells. Immunostaining demonstrated that AT1R was localized on the plasma membrane in wild-type, but intracellularly in NOX1-deficient cells. Immunohistochemistry and immunoblotting showed a decreased expression of AT1R in the aorta of NOX1-deficient mice. To investigate the basis of the abnormal AT1R targeting, we studied caveolin expression and phosphorylation. The amounts of total caveolin and of caveolae were not different in NOX1-deficient mice, but a marked decrease occurred in the phosphorylated form of caveolin. Exogenous H(2)O(2) or transfection of a NOX1 plasmid restored AngII responses in NOX1-deficient cells. Based on these findings, we propose that NOX1-derived reactive oxygen species regulate cell-surface expression of AT1R through mechanisms including caveolin phosphorylation. The lack cell-surface AT1R expression in smooth muscle could be involved in the decreased blood pressure in NOX1-deficient mice.
Keywords Angiotensin II/genetics/metabolism — Animals — Aorta/anatomy & histology — Calcium/metabolism — Caveolins/metabolism — Cells, Cultured — Mice — Mice, Inbred C57BL — Mice, Knockout — Muscle, Smooth, Vascular/cytology/metabolism — Myocytes, Smooth Muscle/cytology/metabolism — NADH, NADPH Oxidoreductases/deficiency/genetics — Phosphorylation — Reactive Oxygen Species/metabolism — Receptor, Angiotensin, Type 1/genetics/metabolism — Signal Transduction/physiology
Identifiers
PMID: 19309260
Full text
Article (Published version) (688 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.liebertonline.com/doi/full/10.1089/ars.2009.2584
Structures
Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme
Research groups Radicaux libres et cellules souches embryonnaires (60)
Obésité et syndrome métabolique (803)
Groupe Schaller Karl-Lothard (neurochirurgie) (851)
Citation
(ISO format) 		BASSET, Olivier et al. NADPH oxidase 1 deficiency alters caveolin phosphorylation and angiotensin II-receptor localization in vascular smooth muscle. In: Antioxidants and Redox Signaling, 2009, vol. 11, n° 10, p. 2371-84. https://archive-ouverte.unige.ch/unige:5405

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Deposited on : 2010-03-12

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