Low- and high-density lipoproteins modulate function, apoptosis, and proliferation of primary human and murine pancreatic beta-cells

Rütti, Sabine; Ehses, Jan A.; Sibler, Rahel A.; Prazak, Richard; Rohrer, Lucia; Georgopoulos, Spiros; Meier, Daniel T.; Niclauss, Nadja; Berney, Thierry; Donath, Marc Y.; von Eckardstein, Arnold

doi:10.1210/en.2009-0252

Advanced search

Browse by...

Deposit

Highlights

More informations

Title		Low- and high-density lipoproteins modulate function, apoptosis, and proliferation of primary human and murine pancreatic beta-cells
Authors		Rütti, Sabine Ehses, Jan A. Sibler, Rahel A. Prazak, Richard Rohrer, Lucia Georgopoulos, Spiros Meier, Daniel T. Niclauss, Nadja Berney, Thierry Donath, Marc Y. von Eckardstein, Arnold show all authors [1 - 11]
Published in		Endocrinology. 2009, vol. 150, no. 10, p. 4521-30
Abstract		A low high-density lipoprotein (HDL) plasma concentration and the abundance of small dense low-density lipoproteins (LDL) are risk factors for developing type 2 diabetes. We therefore investigated whether HDL and LDL play a role in the regulation of pancreatic islet cell apoptosis, proliferation, and secretory function. Isolated mouse and human islets were exposed to plasma lipoproteins of healthy human donors. In murine and human beta-cells, LDL decreased both proliferation and maximal glucose-stimulated insulin secretion. The comparative analysis of beta-cells from wild-type and LDL receptor-deficient mice revealed that the inhibitory effect of LDL on insulin secretion but not proliferation requires the LDL receptor. HDL was found to modulate the survival of both human and murine islets by decreasing basal as well as IL-1beta and glucose-induced apoptosis. IL-1beta-induced beta-cell apoptosis was also inhibited in the presence of either the delipidated protein or the deproteinated lipid moieties of HDL, apolipoprotein A1 (the main protein component of HDL), or sphingosine-1-phosphate (a bioactive sphingolipid mostly carried by HDL). In murine beta-cells, the protective effect of HDL against IL-1beta-induced apoptosis was also observed in the absence of the HDL receptor scavenger receptor class B type 1. Our data show that both LDL and HDL affect function or survival of beta-cells and raise the question whether dyslipidemia contributes to beta-cell failure and hence the manifestation and progression of type 2 diabetes mellitus.
Keywords		Animals — Antigens, CD95/metabolism — Apolipoprotein A-I/metabolism — Apoptosis — CASP8 and FADD-Like Apoptosis Regulating Protein/metabolism — Cell Proliferation — Cell Survival — Cells, Cultured — Female — Glucose/metabolism — Humans — Insulin/secretion — Insulin-Secreting Cells/physiology — Interleukin-1beta/metabolism — Lipoproteins, HDL/physiology — Lipoproteins, LDL/physiology — Lysophospholipids/metabolism — Male — Mice — Mice, Inbred C57BL — Mice, Knockout — Middle Aged — Nitric Oxide Synthase Type II/metabolism — Receptors, LDL/metabolism — Scavenger Receptors, Class B/metabolism — Sphingosine/analogs & derivatives/metabolism
Identifiers		DOI: 10.1210/en.2009-0252 PMID: 19628574
Full text		Article (Published version) (431 Kb) - Limited access to UNIGE Other version: http://endo.endojournals.org/cgi/content/full/150/10/4521
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie
Research group		La transplantation d'îlots de Langerhans (623)
Citation (ISO format)		RÜTTI, Sabine et al. Low- and high-density lipoproteins modulate function, apoptosis, and proliferation of primary human and murine pancreatic beta-cells. In: Endocrinology, 2009, vol. 150, n° 10, p. 4521-30. doi: 10.1210/en.2009-0252 https://archive-ouverte.unige.ch/unige:5338