The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner

Fujita, Toshitsugu; Piuz, Isabelle; Schlegel, Werner

doi:10.1016/j.febslet.2009.07.050

Scientific article

English

The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner

Contributors

Fujita, Toshitsugu; Piuz, Isabelle; Schlegel, Werner

Published in

FEBS letters, vol. 583, no. 17, p. 2893-2898

Publication date

2009

Abstract

We examined whether transcription elongation factors control constitutive transcription of the histone H1(0) and GAPDH genes. Chromatin immunoprecipitation demonstrated positive transcription elongation factor b (P-TEFb) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) present together with RNA polymerase II (pol II) throughout the histone H1(0) gene, whereas negative elongation factor (NELF) was confined to the 5' region. Contrarily, DSIF, NELF and pol II were confined to the 5' region on the GAPDH. Inhibition of those factors affected the constitutive transcription of the histone H1(0) gene but not the GAPDH gene. Thus, NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner.

Keywords

Animals
Cell Line, Tumor
Gene Expression Regulation
Glyceraldehyde-3-Phosphate Dehydrogenases/genetics/metabolism
Histones/genetics/metabolism
Nuclear Proteins/genetics/metabolism
Positive Transcriptional Elongation Factor B/genetics/metabolism
RNA Polymerase II/metabolism
Rats
Transcription Factors/genetics/metabolism
Transcription, Genetic
Transcriptional Elongation Factors/genetics/metabolism

UNIGE affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

Citation (ISO format)

FUJITA, Toshitsugu, PIUZ, Isabelle, SCHLEGEL, Werner. The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner. In: FEBS letters, 2009, vol. 583, n° 17, p. 2893–2898. doi: 10.1016/j.febslet.2009.07.050

Article (Published version)

Identifiers

PID : unige:5320
DOI : 10.1016/j.febslet.2009.07.050
PMID : 19654008

Journal ISSN

0014-5793

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02/03/2010 16:37:00

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02/03/2010 16:37:00

Update

14/03/2023 15:24:35

Status update

14/03/2023 15:24:35

Last indexation

29/10/2024 13:03:13

The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

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Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation