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Scientific article
English

The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner

Published inFEBS letters, vol. 583, no. 17, p. 2893-2898
Publication date2009
Abstract

We examined whether transcription elongation factors control constitutive transcription of the histone H1(0) and GAPDH genes. Chromatin immunoprecipitation demonstrated positive transcription elongation factor b (P-TEFb) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) present together with RNA polymerase II (pol II) throughout the histone H1(0) gene, whereas negative elongation factor (NELF) was confined to the 5' region. Contrarily, DSIF, NELF and pol II were confined to the 5' region on the GAPDH. Inhibition of those factors affected the constitutive transcription of the histone H1(0) gene but not the GAPDH gene. Thus, NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner.

Keywords
  • Animals
  • Cell Line, Tumor
  • Gene Expression Regulation
  • Glyceraldehyde-3-Phosphate Dehydrogenases/genetics/metabolism
  • Histones/genetics/metabolism
  • Nuclear Proteins/genetics/metabolism
  • Positive Transcriptional Elongation Factor B/genetics/metabolism
  • RNA Polymerase II/metabolism
  • Rats
  • Transcription Factors/genetics/metabolism
  • Transcription, Genetic
  • Transcriptional Elongation Factors/genetics/metabolism
Citation (ISO format)
FUJITA, Toshitsugu, PIUZ, Isabelle, SCHLEGEL, Werner. The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner. In: FEBS letters, 2009, vol. 583, n° 17, p. 2893–2898. doi: 10.1016/j.febslet.2009.07.050
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Article (Published version)
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ISSN of the journal0014-5793
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