UNIGE document Scientific Article
previous document  unige:4656  next document
add to browser collection
Title

A non-radioactive method for inexpensive quantitative RT-PCR

Authors
Maggiolini, Marcello
Published in Biological Chemistry. 1999, vol. 380, no. 6, p. 695-7
Abstract We present a novel method for quantitative RT-PCR that involves direct incorporation of digoxigenin-11-dUTP (DIG-dUTP) during amplification of cDNAs, separation of RT-PCR products by agarose gel electrophoresis, Southern transfer to a nylon membrane, and chemiluminescent detection with an anti-DIG antibody. The whole procedure can be done in about a day and has the following advantages: It is highly sensitive, specificity is confirmed by monitoring the size of the RT-PCR product, it is non-radioactive, quantitative, and does not require expensive specialized equipment.
Keywords Base SequenceBlotting, SouthernChemiluminescent MeasurementsDNA PrimersDNA, ComplementaryDeoxyuracil NucleotidesDigoxigenin/analogs & derivativesElectrophoresis, Agar GelHumansRNA, Messenger/analysis/geneticsReceptors, Estrogen/geneticsReference StandardsReverse Transcriptase Polymerase Chain Reaction/methodsSensitivity and SpecificityTumor Cells, Cultured
Identifiers
PMID: 10430034
Full text
Article (Published version) (50 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
MAGGIOLINI, Marcello, DONZE, Olivier, PICARD, Didier. A non-radioactive method for inexpensive quantitative RT-PCR. In: Biological Chemistry, 1999, vol. 380, n° 6, p. 695-7. https://archive-ouverte.unige.ch/unige:4656

226 hits

2 downloads

Update

Deposited on : 2009-12-10

Export document
Format :
Citation style :