Scientific article

An in vitro model of radiation-induced craniofacial bone growth inhibition

Published inThe Journal of craniofacial surgery, vol. 18, no. 5, p. 1044-1050
Publication date2007

Radiation-induced craniofacial bone growth inhibition is a consequence of therapeutic radiation in the survivors of pediatric head and neck cancer. Previously, the infant rabbit orbitozygomatic complex (OZC) was established as a reliable animal model. The purpose of this study was to develop a cell culture model from the rabbit OZC to study the effects of radiation in the craniofacial skeleton. Infant (7-week-old) New Zealand white rabbits were used in this study. Periostea from both OZC were harvested in sterile conditions, introduced into cell culture by way of sequential digestion, and subcultured at confluence. Cultures were analyzed for cellular proliferation (methylthiazoletetrazolium assay), alkaline phosphatase activity, collagen type I expression, and mineralization. Electron microscopy was performed to reveal the in vitro ultrastructure. Subsequently, rabbits were irradiated with sham or 15 Gy radiation, and cell cultures were developed and analyzed for cell numbers. Cell cultures, grown from OZC periostea, expressed osteoblast-like phenotype, with high alkaline phosphatase activity, collagen type 1 expression, and mineralization in an osteogenic environment. Electron microscopy confirmed the characteristic ultrastructural features of osteogenesis in vitro. Finally, significantly (P < 0.01) fewer cells were obtained from animals treated with 15 Gy radiation compared with those from control animals.A primary cell culture with osteoblast-like cellular phenotype was developed from infant rabbit OZC periosteum. This cell culture system responded to in vivo administered radiation by a significant decrease in cell numbers. This in vitro model will be subsequently used to study the cellular mechanisms of radiation and radioprotection in craniofacial osteoblast-like cells.

  • Animals
  • Cell Culture Techniques
  • Cell Proliferation/radiation effects
  • Facial Bones/growth & development/radiation effects
  • Head and Neck Neoplasms/radiotherapy
  • Male
  • Models, Animal
  • Orbit/cytology/radiation effects
  • Periosteum/radiation effects
  • Rabbits
  • Radiation Injuries/complications
  • Zygoma/cytology/radiation effects
Citation (ISO format)
GEVORGYAN, Artur et al. An in vitro model of radiation-induced craniofacial bone growth inhibition. In: The Journal of craniofacial surgery, 2007, vol. 18, n° 5, p. 1044–1050. doi: 10.1097/scs.0b013e31814c916f
Main files (1)
Article (Published version)
ISSN of the journal1049-2275

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