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Title

Characterisation of the influences of aspirin-acetylation and glycation on human plasma proteins

Authors
Priego-Capote, Feliciano
Published in Journal of Proteomics. 2015, vol. 114, p. 125-135
Abstract The competition effect between aspirin-mediated acetylation and protein glycation has been a matter of concern for decades. However, the exact interactions between these two post-translational modifications are still not well understood. Several efforts have been made to explain how aspirin prevents glycation, but the influence of prior protein glycation on the action of aspirin has never been investigated. This study involved qualitative and quantitative analyses to: 1) identify acetylated and glycated proteins; 2) quantify rates of acetylation and glycation; and 3) elucidate the common modification sites. Human plasma was incubated with 30mM glucose and then 500μM aspirin. A label-free mass spectrometry approach indicated an increase in the acetylation level after this sequential glucose-then-aspirin incubation; these results were also confirmed by western blot. Interestingly, for several proteins, decreases in glycation levels were evidenced after aspirin incubation. The common modification sites, where both acetylation and glycation took place, were also identified. The influence that glycation and acetylation processes have on each other could reflect conformational changes induced by glucose and aspirin. In future studies, in order to better understand the interactions between these two PTMs, we intend to apply this strategy to other blood compartments and to diabetic patients.
Identifiers
PMID: 25464367
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Research group Geneva Platelet Group (13)
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FINAMORE, Francesco et al. Characterisation of the influences of aspirin-acetylation and glycation on human plasma proteins. In: Journal of Proteomics, 2015, vol. 114, p. 125-135. https://archive-ouverte.unige.ch/unige:44356

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Deposited on : 2015-01-05

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