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Novel thermosensitive chitosan hydrogels: in vivo evaluation

Osorio-da Cruz, Suzanne
Published in Journal of Biomedical Materials Research. A. 2009, vol. 91, no. 2, p. 324-330
Abstract Chitosan is an attractive biopolymer for the preparation of hydrogels. Its unique combination of biocompatibility, biodegradability, bioadhesivity, and tissue-promoting abilities allows pharmaceutical applications. We investigated novel thermosensitive hydrogels based on chitosan homogeneously reacetylated to a deacetylation degree of about 50%, combined with selected polyols or polyoses such as trehalose, a nontoxic polysaccharide. The latter, a gel-inducing and lyoprotective agent enabled the formulation to be lyophilized and rehydrated without affecting the thermosensitive behavior. This made possible long-term storage and promoted its use in a clinical setup. The thermally induced sol-gel transition allowed injectability and in situ setting. Rheological characterization revealed that storage moduli could be increased by one decade by increasing the chitosan concentration from 1.4 to 2.2% (w/w). Evaluation in vivo provided evidence of in situ implant formation in subcutaneous tissue of Sprague-Dawley rats and permanence for up to 3 months. Histopathological analysis demonstrated a mild, chronic, inflammatory reaction that disappeared with the complete absorption of the gel implant over a few months period. Such in situ forming hydrogels could be advantageous for specific applications in drug delivery and tissue engineering.
PMID: 18980189
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PATOIS, Emilie et al. Novel thermosensitive chitosan hydrogels: in vivo evaluation. In: Journal of Biomedical Materials Research. A, 2009, vol. 91, n° 2, p. 324-330. doi: 10.1002/jbm.a.32211 https://archive-ouverte.unige.ch/unige:4107

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Deposited on : 2009-11-24

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