en
Scientific article
Open access
English

Thapsigargin activates Ca²+ entry both by store-dependent, STIM1/Orai1-mediated, and store-independent, TRPC3/PLC/PKC-mediated pathways in human endothelial cells

Published inCell calcium, vol. 49, no. 2, p. 115-127
Publication date2011
Abstract

The ER Ca²+ sensor STIM1 and the Ca²+ channel Orai1 are key players in store-operated Ca²+ entry (SOCE). In addition, channels from the TRPC family were also shown to be engaged during SOCE, while their precise implication remains controversial. In this study, we investigated the molecular players involved in SOCE triggered by the SERCA pump inhibitor thapsigargin in an endothelial cell line, the EA.hy926. siRNA directed against STIM1 or Orai1 reduced Ca²+ entry by about 50-60%, showing that a large part of the entry is independent from these proteins. Blocking the PLC or the PKC pathway completely abolished thapsigargin-induced Ca²+ entry in cells depleted from STIM1 and/or Orai1. The phorbol ester PMA or the DAG analog OAG restored the Ca²+ entry inhibited by PLC blockers, showing an involvement of PLC/PKC pathway in SOCE. Using pharmacological inhibitors or siRNA revealed that the PKCeta is required for Ca²+ entry, and pharmacological inhibition of the tyrosine kinase Src also reduced Ca²+ entry. TRPC3 silencing diminished the entry by 45%, while the double STIM1/TRPC3 invalidation reduced Ca²+ entry by more than 85%. Hence, in EA.hy926 cells, TG-induced Ca²+ entry results from the activation of the STIM1/Orai1 machinery, and from the activation of TRPC3.

Keywords
  • Calcium/metabolism
  • Calcium Channels/genetics/metabolism
  • Cell Line
  • Endothelial Cells/enzymology/metabolism
  • Fura-2/pharmacology
  • Humans
  • Membrane Proteins/genetics/metabolism
  • Neoplasm Proteins/genetics/metabolism
  • Phorbol Esters/pharmacology
  • Protein Kinase C/antagonists & inhibitors/metabolism
  • RNA Interference
  • RNA, Small Interfering/metabolism
  • Signal Transduction
  • TRPC Cation Channels/genetics/metabolism
  • Thapsigargin/pharmacology
  • Type C Phospholipases/antagonists & inhibitors/metabolism
  • Src-Family Kinases/antagonists & inhibitors/metabolism
Citation (ISO format)
ANTIGNY, Fabrice et al. Thapsigargin activates Ca²+ entry both by store-dependent, STIM1/Orai1-mediated, and store-independent, TRPC3/PLC/PKC-mediated pathways in human endothelial cells. In: Cell calcium, 2011, vol. 49, n° 2, p. 115–127. doi: 10.1016/j.ceca.2010.12.001
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0143-4160
595views
537downloads

Technical informations

Creation07/22/2014 3:11:00 PM
First validation07/22/2014 3:11:00 PM
Update time03/14/2023 9:46:03 PM
Status update03/14/2023 9:46:03 PM
Last indexation05/02/2024 3:33:10 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack