

Other version: http://www.jbc.org/content/280/46/38583.long
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A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell adhesion complex |
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Published in | The Journal of biological chemistry. 2005, vol. 280, no. 46, p. 38583-91 | |
Abstract | Immediately prior to invasion Toxoplasma gondii tachyzoites release a large number of micronemal proteins (TgMICs) that participate in host cell attachment and penetration. The TgMIC4-MIC1-MIC6 complex was the first to be identified in T. gondii and has been recently shown to be critical in invasion. This study establishes that the N-terminal thrombospondin type I repeat-like domains (TSR1-like) from TgMIC1 function as an independent adhesin as well as promoting association with TgMIC4. Using the newly solved three-dimensional structure of the C-terminal domain of TgMIC1 we have identified a novel Galectin-like fold that does not possess carbohydrate binding properties and redefines the architecture of TgMIC1. Instead, the TgMIC1 Galectin-like domain interacts and stabilizes TgMIC6, which provides the basis for a highly specific quality control mechanism for successful exit from the early secretory compartments and for subsequent trafficking of the complex to the micronemes. | |
Keywords | Animals — Blotting, Western — Carbohydrates/chemistry — Cell Adhesion — Cell Adhesion Molecules/chemistry/metabolism/physiology — Cloning, Molecular — Endoplasmic Reticulum/metabolism — Escherichia coli/metabolism — Fluorescent Antibody Technique, Indirect — Galectins/chemistry — Golgi Apparatus/metabolism — Humans — Immunoprecipitation — Magnetic Resonance Spectroscopy — Microscopy, Confocal — Microscopy, Fluorescence — Models, Biological — Molecular Conformation — Neoplasm Invasiveness — Pichia/metabolism — Protein Binding — Protein Conformation — Protein Folding — Protein Structure, Secondary — Protein Structure, Tertiary — Protein Transport — Protozoan Proteins/chemistry/metabolism/physiology — Thrombospondins/metabolism — Toxoplasma/metabolism — Transfection | |
Identifiers | PMID: 16166092 | |
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![]() ![]() Other version: http://www.jbc.org/content/280/46/38583.long |
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Structures | ||
Research group | Biologie d'un parasite intracellulaire obligatoire (773) | |
Citation (ISO format) | SAOUROS, Savvas et al. A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell adhesion complex. In: The Journal of biological chemistry, 2005, vol. 280, n° 46, p. 38583-91. doi: 10.1074/jbc.C500365200 https://archive-ouverte.unige.ch/unige:3971 |