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A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell adhesion complex

Saouros, Savvas
Edwards-Jones, Bryn
Reiss, Matthias
Sawmynaden, Kovilen
Cota, Ernesto
Simpson, Peter
Dowse, Timothy J.
Jäkle, Ursula
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Published in The Journal of biological chemistry. 2005, vol. 280, no. 46, p. 38583-91
Abstract Immediately prior to invasion Toxoplasma gondii tachyzoites release a large number of micronemal proteins (TgMICs) that participate in host cell attachment and penetration. The TgMIC4-MIC1-MIC6 complex was the first to be identified in T. gondii and has been recently shown to be critical in invasion. This study establishes that the N-terminal thrombospondin type I repeat-like domains (TSR1-like) from TgMIC1 function as an independent adhesin as well as promoting association with TgMIC4. Using the newly solved three-dimensional structure of the C-terminal domain of TgMIC1 we have identified a novel Galectin-like fold that does not possess carbohydrate binding properties and redefines the architecture of TgMIC1. Instead, the TgMIC1 Galectin-like domain interacts and stabilizes TgMIC6, which provides the basis for a highly specific quality control mechanism for successful exit from the early secretory compartments and for subsequent trafficking of the complex to the micronemes.
Keywords AnimalsBlotting, WesternCarbohydrates/chemistryCell AdhesionCell Adhesion Molecules/chemistry/metabolism/physiologyCloning, MolecularEndoplasmic Reticulum/metabolismEscherichia coli/metabolismFluorescent Antibody Technique, IndirectGalectins/chemistryGolgi Apparatus/metabolismHumansImmunoprecipitationMagnetic Resonance SpectroscopyMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalMolecular ConformationNeoplasm InvasivenessPichia/metabolismProtein BindingProtein ConformationProtein FoldingProtein Structure, SecondaryProtein Structure, TertiaryProtein TransportProtozoan Proteins/chemistry/metabolism/physiologyThrombospondins/metabolismToxoplasma/metabolismTransfection
PMID: 16166092
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Research group Biologie d'un parasite intracellulaire obligatoire (773)
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SAOUROS, Savvas et al. A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell adhesion complex. In: The Journal of biological chemistry, 2005, vol. 280, n° 46, p. 38583-91. doi: 10.1074/jbc.C500365200 https://archive-ouverte.unige.ch/unige:3971

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Deposited on : 2009-11-05

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