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SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown

Turgay, Yagmur
Champion, Lysie
Balazs, Csaba
Held, Michael
Toso, Alberto
Gerlich, Daniel W
Kutay, Ulrike
Published in The Journal of cell biology. 2014, vol. 204, no. 7, p. 1099-109
Abstract SUN proteins reside in the inner nuclear membrane and form complexes with KASH proteins of the outer nuclear membrane that connect the nuclear envelope (NE) to the cytoskeleton. These complexes have well-established functions in nuclear anchorage and migration in interphase, but little is known about their involvement in mitotic processes. Our analysis demonstrates that simultaneous depletion of human SUN1 and SUN2 delayed removal of membranes from chromatin during NE breakdown (NEBD) and impaired the formation of prophase NE invaginations (PNEIs), similar to microtubule depolymerization or down-regulation of the dynein cofactors NudE/EL. In addition, overexpression of dominant-negative SUN and KASH constructs reduced the occurrence of PNEI, indicating a requirement for functional SUN-KASH complexes in NE remodeling. Codepletion of SUN1/2 slowed cell proliferation and resulted in an accumulation of morphologically defective and disoriented mitotic spindles. Quantification of mitotic timing revealed a delay between NEBD and chromatin separation, indicating a role of SUN proteins in bipolar spindle assembly and mitotic progression.
Keywords Carrier Proteins/metabolismCell ProliferationChromatin/metabolismHeLa CellsHumansIntracellular Signaling Peptides and Proteins/physiologyKineticsMembrane Proteins/physiologyMicroscopy, FluorescenceMicrotubule-Associated Proteins/physiologyNuclear Envelope/metabolismNuclear Proteins/physiologyProphaseSpindle Apparatus/metabolism/ultrastructureTime-Lapse Imaging
PMID: 24662567
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Research group Groupe Meraldi Patrick (physiologie cellulaire et métabolisme) (922)
Swiss National Science Foundation: Investigating the global activities of kinetochores during human cell division
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TURGAY, Yagmur et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. In: The Journal of cell biology, 2014, vol. 204, n° 7, p. 1099-109. doi: 10.1083/jcb.201310116 https://archive-ouverte.unige.ch/unige:38574

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Deposited on : 2014-07-09

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