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Title

A novel mechanism for the initiation of Tacaribe arenavirus genome replication

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Published in Journal of Virology. 1990, vol. 64, no. 12, p. 6196-203
Abstract The ends of arenavirus genome and antigenome RNAs are highly conserved and where determined directly, always contain a 3' G (referred to as position +1). However, primers extended to the 5' ends of Tacaribe virus genomes and antigenomes extend to position -1. When genomes and antigenomes are annealed either inter or intramolecularly and treated with RNase A or T1, there appears to be a single unpaired G at the 5' ends of the hybrids. A single extra G is also found by cloning the 5' ends of S antigenomes, and studies with capping enzyme detect (p)ppG at the 5' ends of genome and antigenome chains. A model is proposed in which genome replication initiates with pppGpC to create the nontemplated extra G. In contrast, the nontemplated bases at the 5' ends of the N mRNAs, which extend to positions -1 to -5, were found to be capped and also heterogeneous in sequence.
Keywords AnimalsArenaviridae/geneticsBase SequenceCell LineCloning, MolecularGenes, ViralMolecular Sequence DataNucleic Acid HybridizationOligonucleotide ProbesRNA Caps/geneticsRNA Viruses/geneticsRNA, Messenger/geneticsTranscription, Genetic
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PMID: 2243392
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Article (Published version) (2.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Virologie moléculaire (275)
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GARCIN, Dominique, KOLAKOFSKY, Daniel. A novel mechanism for the initiation of Tacaribe arenavirus genome replication. In: Journal of Virology, 1990, vol. 64, n° 12, p. 6196-203. https://archive-ouverte.unige.ch/unige:37928

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Deposited on : 2014-06-18

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