en
Scientific article
English

A novel mechanism for the initiation of Tacaribe arenavirus genome replication

Published inJournal of virology, vol. 64, no. 12, p. 6196-6203
Publication date1990
Abstract

The ends of arenavirus genome and antigenome RNAs are highly conserved and where determined directly, always contain a 3' G (referred to as position +1). However, primers extended to the 5' ends of Tacaribe virus genomes and antigenomes extend to position -1. When genomes and antigenomes are annealed either inter or intramolecularly and treated with RNase A or T1, there appears to be a single unpaired G at the 5' ends of the hybrids. A single extra G is also found by cloning the 5' ends of S antigenomes, and studies with capping enzyme detect (p)ppG at the 5' ends of genome and antigenome chains. A model is proposed in which genome replication initiates with pppGpC to create the nontemplated extra G. In contrast, the nontemplated bases at the 5' ends of the N mRNAs, which extend to positions -1 to -5, were found to be capped and also heterogeneous in sequence.

Keywords
  • Animals
  • Arenaviridae/genetics
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Genes, Viral
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • RNA Caps/genetics
  • RNA Viruses/genetics
  • RNA, Messenger/genetics
  • Transcription, Genetic
Citation (ISO format)
GARCIN, Dominique, KOLAKOFSKY, Daniel. A novel mechanism for the initiation of Tacaribe arenavirus genome replication. In: Journal of virology, 1990, vol. 64, n° 12, p. 6196–6203.
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Article (Published version)
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ISSN of the journal0022-538X
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