Synthetic multifunctional pores: deletion and inversion of anion/cation selectivity using pM and pH

Sakai, Naomi; Sorde, Nathalie; Das, Gopal; Perrottet, Philippe; Gerard, David; Matile, Stefan

doi:10.1039/b210604c

Scientific article

English

Synthetic multifunctional pores: deletion and inversion of anion/cation selectivity using pM and pH

Contributors

Sakai, Naomi

; Sorde, Nathalie; Das, Gopal; Perrottet, Philippe; Gerard, David; Matile, Stefan

Published in

Organic & biomolecular chemistry, vol. 1, no. 7, p. 1226-1231

Publication date

2003

Abstract

We report the characterization of multifunctional rigid-rod [beta]-barrel ion channels with either internal aspartates or arginine–histidine dyads by planar bilayer conductance experiments. Barrels with internal aspartates form cation selective, large, unstable and ohmic barrel-stave (rather than toroidal) pores; addition of magnesium cations nearly deletes cation selectivity and increases single-channel stability. Barrels with internal arginine–histidine dyads form cation selective (P[K+]/P[Cl–]= 2.1), small and ohmic ion channels with superb stability (single-channel lifetime > 20 seconds). Addition of "protons" results in inversion of anion/cation selectivity (P[Cl–]/P[K+]= 3.8); addition of an anionic guest (HPTS) results in the blockage of anion selective but not cation selective channels. These results suggest that specific, internal counterion immobilization, here magnesium (but not sodium or potassium) cations by internal aspartates and inorganic phosphates by internal arginines (but not histidines), provides access to synthetic multifunctional pores with attractive properties.

UNIGE affiliation entities

UNIGE research groups

Groupe Gülaçar

Citation (ISO format)

SAKAI, Naomi et al. Synthetic multifunctional pores: deletion and inversion of anion/cation selectivity using pM and pH. In: Organic & biomolecular chemistry, 2003, vol. 1, n° 7, p. 1226–1231. doi: 10.1039/b210604c

Article (Published version)

Identifiers

PID : unige:3716
DOI : 10.1039/b210604c

Additional URL for this publication

http://pubs.rsc.org/ej/OB/2003/b210604c.pdf?&Yr=2003&VOLNO=%26nbsp%3B%3Cb%3E1%3C%2Fb%3E&Fp=1226&Ep=1231&JournalCode=OB&Iss=7

Journal ISSN

1477-0520

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684downloads

Creation

06/10/2009 11:58:58

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06/10/2009 11:58:58

Update

14/10/2025 07:07:04

Status update

14/03/2023 15:16:06

Last indexation

16/02/2026 06:55:07

Synthetic multifunctional pores: deletion and inversion of anion/cation selectivity using pM and pH

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

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Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation