UNIGE document Scientific Article
previous document  unige:32605  next document
add to browser collection

De- and repolarization mechanism of flagellar morphogenesis during a bacterial cell cycle

Davis, Nicole J
Cohen, Yaniv
Ozaki, Shogo
Luciano, Jennifer
Guerrero-Ferreira, Ricardo C
Wright, Elizabeth R
show hidden authors show all authors [1 - 10]
Published in Genes and Development. 2013, vol. 27, no. 18, p. 2049-62
Abstract Eukaryotic morphogenesis is seeded with the establishment and subsequent amplification of polarity cues at key times during the cell cycle, often using (cyclic) nucleotide signals. We discovered that flagellum de- and repolarization in the model prokaryote Caulobacter crescentus is precisely orchestrated through at least three spatiotemporal mechanisms integrated at TipF. We show that TipF is a cell cycle-regulated receptor for the second messenger-bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP)-that perceives and transduces this signal through the degenerate c-di-GMP phosphodiesterase (EAL) domain to nucleate polar flagellum biogenesis. Once c-di-GMP levels rise at the G1 → S transition, TipF is activated, stabilized, and polarized, enabling the recruitment of downstream effectors, including flagellar switch proteins and the PflI positioning factor, at a preselected pole harboring the TipN landmark. These c-di-GMP-dependent events are coordinated with the onset of tipF transcription in early S phase and together enable the correct establishment and robust amplification of TipF-dependent polarization early in the cell cycle. Importantly, these mechanisms also govern the timely removal of TipF at cell division coincident with the drop in c-di-GMP levels, thereby resetting the flagellar polarization state in the next cell cycle after a preprogrammed period during which motility must be suspended.
PMID: 24065770
Full text
Article (Published version) (2.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research group Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
(ISO format)
DAVIS, Nicole J et al. De- and repolarization mechanism of flagellar morphogenesis during a bacterial cell cycle. In: Genes and Development, 2013, vol. 27, n° 18, p. 2049-62. https://archive-ouverte.unige.ch/unige:32605

211 hits

1 download


Deposited on : 2013-12-20

Export document
Format :
Citation style :