• Home
  1. Home
  2. Screening and determination of sibutramine in adulterated herbal slimming supplements by HPTLC-UV densitometry
Scientific article
English

Screening and determination of sibutramine in adulterated herbal slimming supplements by HPTLC-UV densitometry

Contributors

Mathon, Caroline; Ankli, Anita; Reich, Eike; Bieri, Stefan; Christen, Philippe

Published in

Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, vol. 31, no. 1, p. 15-20

Publication date

2014

Abstract

The adulteration of herbal supplements is of growing importance, especially when they contain undeclared compounds like sibutramine that are unsafe drugs. Sibutramine was withdrawn from US and European markets in 2010. In this study, an HPTLC-UV densitometric method was developed for the quantification of sibutramine in herbal diet foods. Sample extracts were directly applied onto HPTLC silica gel plates and separated with a mobile phase made of a toluene-methanol mixture. Sibutramine was quantified at 225 nm and its unequivocal identification was confirmed by mass spectrometry using a TLC-MS interface. During two surveys, 52 weight loss supplements obtained via the Internet were screened. Half of those were adulterated with sibutramine at amounts reaching up to 35 mg per capsule. The results of this validated HPTLC method were compared with those obtained by HPLC-UV and HPLC-MS/MS. The concentrations were not significantly different with the three methods.

Keywords

  • Herbal slimming supplements
  • Weight loss
  • Sibutramine
  • Adulteration
  • HPTLC
  • Densitogram

UNIGE affiliation entities

Citation (ISO format)

MATHON, Caroline et al. Screening and determination of sibutramine in adulterated herbal slimming supplements by HPTLC-UV densitometry. In: Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, 2014, vol. 31, n° 1, p. 15–20. doi: 10.1080/19440049.2013.861934

Main files (1)

Article (Accepted version)

Identifiers

Journal ISSN

1944-0057
704views
0downloads

Technical informations

Creation

11/12/2013 10:51:00

First validation

11/12/2013 10:51:00

Update

14/03/2023 20:42:32

Status update

14/03/2023 20:42:32

Last indexation

30/10/2024 15:19:20
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated