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Title

The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution

Authors
James, Nicole
Dubouloz, Frédérique
Werner, Michel
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Published in Molecular and Cellular Biology. 2005, vol. 25, no. 1, p. 488-98
Abstract The Ccr4-Not complex is a conserved global regulator of gene expression, which serves as a regulatory platform that senses and/or transmits nutrient and stress signals to various downstream effectors. Presumed effectors of this complex in yeast are TFIID, a general transcription factor that associates with the core promoter, and Msn2, a key transcription factor that regulates expression of stress-responsive element (STRE)-controlled genes. Here we show that the constitutively high level of STRE-driven expression in ccr4-not mutants results from two independent effects. Accordingly, loss of Ccr4-Not function causes a dramatic Msn2-independent redistribution of TFIID on promoters with a particular bias for STRE-controlled over ribosomal protein gene promoters. In parallel, loss of Ccr4-Not complex function results in an alteration of the posttranslational modification status of Msn2, which depends on the type 1 protein phosphatase Glc7 and its newly identified subunit Bud14. Tests of epistasis as well as transcriptional analyses of Bud14-dependent transcription support a model in which the Ccr4-Not complex prevents activation of Msn2 via inhibition of the Bud14/Glc7 module in exponentially growing cells. Thus, increased activity of STRE genes in ccr4-not mutants may result from both altered general distribution of TFIID and unscheduled activation of Msn2.
Keywords Cell Cycle Proteins/physiologyCross-Linking Reagents/pharmacologyDNA/metabolismDNA-Binding Proteins/physiologyGene Expression RegulationGenotypeGlucose/metabolismImmunoblottingImmunoprecipitationModels, BiologicalMutationNucleic Acid HybridizationPhosphoprotein Phosphatases/metabolismPlasmids/metabolismPromoter Regions, GeneticProtein BindingProtein Phosphatase 1Protein Processing, Post-TranslationalRNA, Messenger/metabolismRibonucleases/physiologySaccharomyces cerevisiae/metabolismSaccharomyces cerevisiae Proteins/metabolism/physiologyTime FactorsTranscription Factor TFIID/chemistryTranscription Factors/physiologyTranscription, GeneticTranscriptional ActivationTwo-Hybrid System Techniques
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PMID: 15601868
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Research group Groupe Collart Martine (microbiologie et médecine moléculaire) (81)
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LENSSEN, Eve et al. The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution. In: Molecular and Cellular Biology, 2005, vol. 25, n° 1, p. 488-98. https://archive-ouverte.unige.ch/unige:30077

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Deposited on : 2013-10-01

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