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Scientific article
English

The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution

Published inMolecular and cellular biology, vol. 25, no. 1, p. 488-498
Publication date2005
Abstract

The Ccr4-Not complex is a conserved global regulator of gene expression, which serves as a regulatory platform that senses and/or transmits nutrient and stress signals to various downstream effectors. Presumed effectors of this complex in yeast are TFIID, a general transcription factor that associates with the core promoter, and Msn2, a key transcription factor that regulates expression of stress-responsive element (STRE)-controlled genes. Here we show that the constitutively high level of STRE-driven expression in ccr4-not mutants results from two independent effects. Accordingly, loss of Ccr4-Not function causes a dramatic Msn2-independent redistribution of TFIID on promoters with a particular bias for STRE-controlled over ribosomal protein gene promoters. In parallel, loss of Ccr4-Not complex function results in an alteration of the posttranslational modification status of Msn2, which depends on the type 1 protein phosphatase Glc7 and its newly identified subunit Bud14. Tests of epistasis as well as transcriptional analyses of Bud14-dependent transcription support a model in which the Ccr4-Not complex prevents activation of Msn2 via inhibition of the Bud14/Glc7 module in exponentially growing cells. Thus, increased activity of STRE genes in ccr4-not mutants may result from both altered general distribution of TFIID and unscheduled activation of Msn2.

Keywords
  • Cell Cycle Proteins/physiology
  • Cross-Linking Reagents/pharmacology
  • DNA/metabolism
  • DNA-Binding Proteins/physiology
  • Gene Expression Regulation
  • Genotype
  • Glucose/metabolism
  • Immunoblotting
  • Immunoprecipitation
  • Models, Biological
  • Mutation
  • Nucleic Acid Hybridization
  • Phosphoprotein Phosphatases/metabolism
  • Plasmids/metabolism
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Phosphatase 1
  • Protein Processing, Post-Translational
  • RNA, Messenger/metabolism
  • Ribonucleases/physiology
  • Saccharomyces cerevisiae/metabolism
  • Saccharomyces cerevisiae Proteins/metabolism/physiology
  • Time Factors
  • Transcription Factor TFIID/chemistry
  • Transcription Factors/physiology
  • Transcription, Genetic
  • Transcriptional Activation
  • Two-Hybrid System Techniques
Citation (ISO format)
LENSSEN, Eve et al. The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution. In: Molecular and cellular biology, 2005, vol. 25, n° 1, p. 488–498. doi: 10.1128/MCB.25.1.488-498.2005
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Article (Published version)
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Identifiers
ISSN of the journal0270-7306
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