en
Scientific article
English

Expression of the basal cell adhesion molecule (B-CAM) in normal and diseased human skin

Published inJournal of cutaneous pathology, vol. 27, no. 3, p. 108-111
Publication date2000
Abstract

The basal cell adhesion molecule (B-CAM) is a 90-kD cell surface glycoprotein with a characteristic immunoglobulin domain structure. The pattern of B-CAM expression in cultured cells suggests that the molecule is associated with a substrate-adherent growth pattern in some lineages. We investigated the expression of B-CAM in normal and diseased human epidermis by means of immunohistochemistry employing a single batch of high-titer mouse monoclonal antibody G253. Snap-frozen biopsy material from normal skin (n = 8), psoriasis (n = 5), contact dermatitis (n = 6), basal cell carcinoma (n = 5) and fetal skin (n = 6) was studied. In normal human skin, B-CAM was found in varying degrees throughout the epidermis with a preference for suprabasal expression, hair follicles were regularly of a B-CAM-positive phenotype. There were no qualitative differences with regard to the B-CAM expression pattern in normal skin in comparison to psoriasis and contact dermatitis. In contrast, fetal skin (15th to 18th week of gestation) was characterized by B-CAM-positive cells in the basal layer of the epidermis as well as in the outer root sheath of hair follicles. Basal cell carcinomas also regularly expressed high levels of B-CAM. A strong B-CAM-positive phenotype can be found in the outer root sheath of hair follicles of adult and fetal human skin as well as in fetal basal keratinocytes.

Keywords
  • Carcinoma, Basal Cell/metabolism/pathology
  • Cell Adhesion Molecules/*metabolism
  • Dermatitis, Contact/metabolism/pathology
  • Embryonic and Fetal Development
  • Fetus
  • Hair Follicle/embryology/metabolism
  • Humans
  • Immunohistochemistry
  • Keratinocytes/metabolism
  • Lutheran Blood-Group System
  • Neoplasm Proteins/*metabolism
  • Psoriasis/metabolism/pathology
  • Skin/embryology/*metabolism/pathology
  • Skin Diseases/*metabolism/pathology
  • Skin Neoplasms/metabolism/pathology
Affiliation Not a UNIGE publication
Citation (ISO format)
BERNEMANN, T. M. et al. Expression of the basal cell adhesion molecule (B-CAM) in normal and diseased human skin. In: Journal of cutaneous pathology, 2000, vol. 27, n° 3, p. 108–111. doi: 10.1034/j.1600-0560.2000.027003108.x
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ISSN of the journal0303-6987
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