Scientific article

TiNOx coatings increase human primary osteoblasts proliferation independently of the substrate: a short report

Published inBio-medical materials and engineering, vol. 22, no. 5, p. 277-281
Publication date2012

Introduction: When applied onto SLA titanium, Titanium Nitride Oxide (TiNOx) coatings are known for their stimulating effect on osteoblast proliferation while maintaining a high degree of differentiation. Objective: To determine whether the stimulating effect of TiNOx coatings is or is not dependent of the metal substrate.Methods: A TiNOx coating was deposited on roughened stainless steel (SS-SLA-TiNOx). The proliferation and differentiation of human primary osteoblasts were determined and compared to that observed on standard SLA titanium. To this end, cell proliferation and gene expression were monitored for a time course of 3 weeks, with or without additional stimulation by 100 nM 1.25(OH)2 vitamin D3. Results: A 1.5 fold increase in the proliferation rate of cells grown on SS-SLA-TiNOx as compared to Ti-SLA surfaces was observed. A high level of differentiation was also noted. Conclusion: TiNOx coatings yield similar proliferation and differentiation rates when applied onto roughened Ti and SS. This might translate into a more effective osseointegration of endosseous implants made of stronger metals than Ti.

  • Alkaline Phosphatase/genetics/metabolism
  • Bone Morphogenetic Protein 2/genetics/metabolism
  • Cell Differentiation/drug effects
  • Cell Proliferation/drug effects
  • Coated Materials, Biocompatible/pharmacology
  • Core Binding Factor Alpha 1 Subunit/genetics/metabolism
  • Humans
  • Osteoblasts/cytology/drug effects
  • Osteopontin/genetics/metabolism
  • Osteoprotegerin/genetics/metabolism
  • Stainless Steel/chemistry
  • Surface Properties
  • Titanium/pharmacology
Citation (ISO format)
RIEDER, Philippe et al. TiNOx coatings increase human primary osteoblasts proliferation independently of the substrate: a short report. In: Bio-medical materials and engineering, 2012, vol. 22, n° 5, p. 277–281. doi: 10.3233/BME-2012-0717
Main files (1)
Article (Published version)
ISSN of the journal0959-2989

Technical informations

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