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Title

Complete absence of the αGal xenoantigen and isoglobotrihexosylceramide in α1,3galactosyltransferase knock-out pigs

Authors
Li, Yunsen
Borsig, Lubor
Millard, Anne-Laure
Karpova, Maria B
Zhou, Dapeng
Published in Xenotransplantation. 2012, vol. 19, no. 3, p. 196-206
Abstract Anti-Galα1,3Galβ-R natural antibodies are responsible for hyperacute rejection in pig-to-primate xenotransplantation. Although the generation of pigs lacking the α1,3galactosyltransferase (GalT) has overcome hyperacute rejection, antibody-mediated rejection is still a problem. It is possible that other enzymes synthesize antigens similar to Galα1,3Gal epitopes that are recognized by xenoreactive antibodies. The glycosphingolipid isoglobotrihexosylceramide (iGb₃) represents such a candidate expressing an alternative Galα1,3Gal epitope. The present work determined whether the terminal Galα1,3Gal disaccharide is completely absent in Immerge pigs lacking the GalT using several different highly sensitive methods.
Keywords Acute DiseaseAnimalsAntigens, Heterophile/immunology/metabolismAorta/cytologyDisaccharides/immunology/metabolismEndothelial Cells/cytology/enzymologyFemaleGalactosyltransferases/genetics/metabolismGene Knockdown Techniques/methodsGlobosides/immunology/metabolismGraft Rejection/genetics/immunology/prevention & controlPrimatesRNA, Messenger/metabolismSwineSwine, MiniatureTransplantation, Heterologous/immunology/methods
Identifiers
PMID: 22702471
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Article (Published version) (474 Kb) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Groupe Seebach Jorg Dieter (transplantation et immunologie) (856)
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(ISO format)
PUGA YUNG, Gisella et al. Complete absence of the αGal xenoantigen and isoglobotrihexosylceramide in α1,3galactosyltransferase knock-out pigs. In: Xenotransplantation, 2012 , vol. 19, n° 3, p. 196-206. https://archive-ouverte.unige.ch/unige:27589

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Deposited on : 2013-04-26

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