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Title

Isolation of rat embryonic stem-like cells: a tool for stem cell research and drug discovery

Authors
Pirondi, S
Chen, B L
Del Vecchio, G
Alessandri, M
Farnedi, A
Pession, A
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Published in Developmental Dynamics. 2011, vol. 240, no. 11, p. 2482-94
Abstract The establishment of rat embryonic stem cells constitutes a precious tool since rat has been extensively used in biomedical research, in particular for the generation of human neurodisease animal models. Up to now only a few studies have described the isolation of rat embryonic stem-like cells. One out of 9 isolated rat embryonic stem-like cell lines (B1-RESC) obtained from a 4.5-day post-coitum blastocyst were extensively characterized and kept in culture for up to 80 passages on feeders with LIF. The stable growth of these cells and the expression of pluripotent markers were confirmed up to a high number of passages in culture, also in the absence of feeders and LIF. B1-RESC expresses the three germ layers markers both in vitro, within differentiating embryoid bodies, and in vivo through teratoma formation. Collectively, the B1-RESC line with a stable near-diploid karyotype can be used as a highly sensitive tool for testing anti-proliferative molecules.
Keywords AnimalsCell Differentiation/genetics/physiologyCell Separation/methodsCells, CulturedDrug Discovery/methodsEmbryonic Stem Cells/cytology/metabolismFemaleGene Expression Regulation, DevelopmentalMiceModels, BiologicalPregnancyRatsRats, Sprague-DawleyStem Cell Research
Identifiers
PMID: 22012593
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Article (Published version) (2.4 MB) - public document Free access
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Research group Mécanismes de différentiation cellulaire des cellules cardiaques (536)
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FERNANDEZ, Marylise et al. Isolation of rat embryonic stem-like cells: a tool for stem cell research and drug discovery. In: Developmental Dynamics, 2011, vol. 240, n° 11, p. 2482-94. https://archive-ouverte.unige.ch/unige:25636

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Deposited on : 2013-01-17

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