Scientific article
Open access

Isolation of rat embryonic stem-like cells: a tool for stem cell research and drug discovery

Published inDevelopmental dynamics, vol. 240, no. 11, p. 2482-2494
Publication date2011

The establishment of rat embryonic stem cells constitutes a precious tool since rat has been extensively used in biomedical research, in particular for the generation of human neurodisease animal models. Up to now only a few studies have described the isolation of rat embryonic stem-like cells. One out of 9 isolated rat embryonic stem-like cell lines (B1-RESC) obtained from a 4.5-day post-coitum blastocyst were extensively characterized and kept in culture for up to 80 passages on feeders with LIF. The stable growth of these cells and the expression of pluripotent markers were confirmed up to a high number of passages in culture, also in the absence of feeders and LIF. B1-RESC expresses the three germ layers markers both in vitro, within differentiating embryoid bodies, and in vivo through teratoma formation. Collectively, the B1-RESC line with a stable near-diploid karyotype can be used as a highly sensitive tool for testing anti-proliferative molecules.

  • Animals
  • Cell Differentiation/genetics/physiology
  • Cell Separation/methods
  • Cells, Cultured
  • Drug Discovery/methods
  • Embryonic Stem Cells/cytology/metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Mice
  • Models, Biological
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley
  • Stem Cell Research
Citation (ISO format)
FERNANDEZ, Marylise et al. Isolation of rat embryonic stem-like cells: a tool for stem cell research and drug discovery. In: Developmental dynamics, 2011, vol. 240, n° 11, p. 2482–2494. doi: 10.1002/dvdy.22761
Main files (1)
Article (Published version)
ISSN of the journal1058-8388

Technical informations

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