Scientific article

Decay-accelerating factor binding determines the entry route of echovirus 11 in polarized epithelial cells

Published inJournal of virology, vol. 85, no. 23, p. 12376-12386
Publication date2011

The interaction between echovirus 11 strain 207 (EV11-207) and decay-accelerating factor (DAF or CD55) at the apical surface of polarized Caco-2 cells results in rapid transport of the virus to tight junctions and in its subsequent uptake. A virus mutant (EV11-207R) which differs at 6 amino acids and whose affinity for DAF is apparently significantly lower remains at the apical surface, from where its uptake occurs. Binding of EV11-207 to DAF and its transport to tight junctions result in a loss of function of the junctions. In contrast, the mutant virus EV11-207R is not transferred to tight junctions, nor does it impair the integrity of these junctions. Cholesterol depletion from the apical membrane leads to DAF aggregation and, presumably, internalization and inhibits infection by EV11-207. However, infection by EV11-207R is significantly less sensitive to cholesterol depletion than infection by EV11-207, confirming the DAF requirement for EV11-207, but not EV11-207R, to infect cells. These data strongly indicate that in the case of infection of polarized epithelial cells by echovirus 11, DAF binding appears be a key determinant in the choice of entry pathway, at least in cell culture.

  • Antigens, CD55/metabolism
  • Caco-2 Cells
  • Cell Polarity
  • Cholesterol/deficiency
  • Enterovirus B, Human/drug effects/metabolism/pathogenicity
  • Enterovirus Infections/metabolism/virology
  • Epithelial Cells/virology
  • Fluorescent Antibody Technique
  • Humans
  • Immunoblotting
  • Protein Binding
  • Tight Junctions/metabolism
  • Virus Internalization/drug effects
  • Virus Replication
  • Beta-Cyclodextrins/pharmacology
Citation (ISO format)
SOBO, Komla et al. Decay-accelerating factor binding determines the entry route of echovirus 11 in polarized epithelial cells. In: Journal of virology, 2011, vol. 85, n° 23, p. 12376–12386. doi: 10.1128/JVI.00016-11
Main files (1)
Article (Published version)
ISSN of the journal0022-538X

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