Immunity to pneumococcal surface proteins in children with community-acquired pneumonia: a distinct pattern of responses to pneumococcal choline-binding protein A.

Posfay Barbe, Clara; Galetto-Lacour, Annick; Grillet, Stéphane; Ochs, M M; Brookes, R H; Kraehenbuhl, J D; Cevey-Macherel, M; Gehri, M; Gervaix, Alain; Siegrist, Claire-Anne

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Title		Immunity to pneumococcal surface proteins in children with community-acquired pneumonia: a distinct pattern of responses to pneumococcal choline-binding protein A.
Authors		Posfay Barbe, Clara Galetto-Lacour, Annick Grillet, Stéphane Ochs, M M Brookes, R H Kraehenbuhl, J D Cevey-Macherel, M Gehri, M Gervaix, Alain Siegrist, Claire-Anne show all authors [1 - 10]
Published in		Clinical Microbiology and Infection. 2011, vol. 17, no. 8, p. 1232-8
Abstract		The aetiological diagnosis of community-acquired pneumonia (CAP) is challenging in children, and serological markers would be useful surrogates for epidemiological studies of pneumococcal CAP. We compared the use of anti-pneumolysin (Ply) antibody alone or with four additional pneumococcal surface proteins (PSPs) (pneumococcal histidine triad D (PhtD), pneumococcal histidine triad E (PhtE), LytB, and pneumococcal choline-binding protein A (PcpA)) as serological probes in children hospitalized with CAP. Recent pneumococcal exposure (positive blood culture for Streptococcus pneumoniae, Ply(+) blood PCR finding, and PSP seroresponse) was predefined as supporting the diagnosis of presumed pneumococcal CAP (P-CAP). Twenty-three of 75 (31%) children with CAP (mean age 33.7 months) had a Ply(+) PCR finding and/or a ≥ 2-fold increase of antibodies. Adding seroresponses to four PSPs identified 12 additional patients (35/75, 45%), increasing the sensitivity of the diagnosis of P-CAP from 0.44 (Ply alone) to 0.94. Convalescent anti-Ply and anti-PhtD antibody titres were significantly higher in P-CAP than in non P-CAP patients (446 vs. 169 ELISA Units (EU)/mL, p 0.031, and 189 vs. 66 EU/mL, p 0.044), confirming recent exposure. Acute anti-PcpA titres were three-fold lower (71 vs. 286 EU/mL, p <0.001) in P-CAP children. Regression analyses confirmed a low level of acute PcpA antibodies as the only independent predictor (p 0.002) of P-CAP. Novel PSPs facilitate the demonstration of recent pneumococcal exposure in CAP children. Low anti-PcpA antibody titres at admission distinguished children with P-CAP from those with CAP with a non-pneumococcal origin.
Keywords		Adhesins, Bacterial/immunology — Antibodies, Bacterial/blood — Bacterial Proteins/genetics/immunology — Carrier Proteins/immunology — Child, Preschool — Community-Acquired Infections/diagnosis/immunology — Humans — Lipoproteins/immunology — Membrane Proteins/immunology — Pneumonia, Pneumococcal/diagnosis/immunology — Sensitivity and Specificity — Streptococcus pneumoniae/immunology — Streptolysins/genetics/immunology
Identifiers		DOI: 10.1111/j.1469-0691.2010.03389.x PMID: 21040158
Full text		Article (Published version) (136 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de pédiatrie
Research groups		Centre de Vaccinologie et d'Immunologie néonatale (177) Etiologie des pneumonies et marqueurs inflammatoires chez l'enfant fébrile (183) Groupe Posfay-Barbe Clara (pédiatrie générale) (853)
Citation (ISO format)		POSFAY BARBE, Clara et al. Immunity to pneumococcal surface proteins in children with community-acquired pneumonia: a distinct pattern of responses to pneumococcal choline-binding protein A. In: Clinical Microbiology and Infection, 2011, vol. 17, n° 8, p. 1232-8. https://archive-ouverte.unige.ch/unige:25174