UNIGE document Scientific Article
previous document  unige:24880  next document
add to browser collection
Title

Detecting rejection after mouse Islet transplantation utilizing islet protein-stimulated ELISPOT.

Authors
Pawlick, Rena
Edgar, Ryan
Davis, Joy
McCall, Michael
show hidden authors show all authors [1 - 10]
Published in Cell Transplantation. 2011, vol. 20, no. 6, p. 955-62
Abstract Improved posttransplant monitoring and on-time detection of rejection could improve islet transplantation outcome. The present study explored the possibility of detecting harmful events after mouse islet transplantation measuring the immune responsiveness against islet extracts. Mouse islet transplantations were performed using various donor/recipient combinations, exploring autoimmune (NOD/SCID to NOD, n = 6) and alloimmune events (C57BL/6 to BALB/c, n = 20), a combination of both (C57BL/6 to NOD, n = 8), the absence of both (BALB/c to BALB/c, n = 21), or naive, nontransplanted control mice (n = 14). The immune reactivity was measured by ELISPOT, looking at the ex vivo release of IFN-γ from splenocytes stimulated by islet donor extracts (sonicated islets). The immune reactivity was not altered in the syngeneic and autoimmune models, demonstrating similar levels as nontransplanted controls (p = 0.46 and p = 0.6). Conversely, the occurrence of an allogeneic rejection alone or in combination to autoimmunity was associated to an increase in the level of immune reactivity (p = 0.023 and p = 0.003 vs. respective controls). The observed increase was transient and lost in the postrejection period or after treatment with CTLA4-Ig. Overall, allogeneic rejection was associated to a transient increase in the reactivity of splenocytes against islet proteins. Such a strategy has the potential to improve islet graft monitoring in human and should be further explored.
Keywords AnimalsAutoimmunity/physiologyCytokines/metabolismDiabetes Mellitus, Experimental/therapyDisease Models, AnimalEnzyme-Linked Immunospot AssayFemaleGraft Rejection/immunologyImmunosuppressive Agents/pharmacologyInterferon-gamma/metabolismIslets of Langerhans Transplantation/immunologyMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred NODROC CurveSpleen/cytologyTransplantation, AutologousTransplantation, Homologous
Identifiers
PMID: 21054945
Full text
Article (Published version) (257 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Transplantation et hépatologie (905)
Hépatologie chirurgicale (327)
La transplantation d'îlots de Langerhans (623)
Citation
(ISO format)
TOSO, Christian et al. Detecting rejection after mouse Islet transplantation utilizing islet protein-stimulated ELISPOT. In: Cell Transplantation, 2011, vol. 20, n° 6, p. 955-62. https://archive-ouverte.unige.ch/unige:24880

192 hits

0 download

Update

Deposited on : 2013-01-02

Export document
Format :
Citation style :