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Title

Maturation méiotique de l'ovocyte de souris : rôles des actines cytoplasmiques

Author
Brockmann-Colomb, Céline
Directors
Defense Thèse de doctorat : Univ. Genève, 2008 - Sc. 3975 - 2008/04/22
Abstract The mammalian oocyte undergoes two asymmetric cleavages to accomplish meiosis I and II. The actin cytoskeleton is the key player in establishing this asymmetry. Using monoclonal antibodies we have compared the distribution of β- and [upsilon]-cytoplasmic actins (CYAs) in mouse oocytes and early embryos. We show that β-CYA preferentially localized to the cleavage furrow, contractile ring, and cell-cell contact regions. [Upsilon]-CYA showed a widespread cortical localization and was specifically excluded from cell adhesion areas. Oocyte microinjections of isoform-specific perturbators were conducted to modulate specifically and separately isoform functions. Our results suggest that [upsilon]-CYA holds a specific role in the establishment of asymmetry in meiosis I; both β- and [upsilon]-CYA together, appear to participate in the formation and the cortical anchorage of the second meiotic spindle. Our results reveal functional diversity for the two highly conserved non-muscle actin isoforms and provide evidence for an involvement of [upsilon]-CYA in reduced fertility.
Keywords ActinMeiosisCocyteCytoskeleton
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URN: urn:nbn:ch:unige-21574
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BROCKMANN-COLOMB, Céline. Maturation méiotique de l'ovocyte de souris : rôles des actines cytoplasmiques. Université de Genève. Thèse, 2008. https://archive-ouverte.unige.ch/unige:2157

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Deposited on : 2009-06-24

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