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Approaches to study differentiation and repair of human airway epithelial cells

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Published in Methods in Molecular Biology. 2011, vol. 742, p. 173-85
Abstract One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.
Keywords AnimalsCell Communication/physiologyCell Culture TechniquesCell Differentiation/physiologyEpithelial Cells/cytology/metabolismHumansImmunohistochemistryInterleukin-8/analysis/biosynthesisMiceModels, BiologicalMucins/analysis/biosynthesisResearch DesignRespiratory Mucosa/cytology/metabolismWound Healing/physiology
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PMID: 21547732
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Research group Mucoviscidose et jonctions GAP (229)
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CRESPIN, Sophie et al. Approaches to study differentiation and repair of human airway epithelial cells. In: Methods in Molecular Biology, 2011, vol. 742, p. 173-85. https://archive-ouverte.unige.ch/unige:21534

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Deposited on : 2012-06-07

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