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Title

Approaches to study differentiation and repair of human airway epithelial cells
Authors
Crespin, Sophie
Bacchetta, Marc
Huang, Song
Dudez, Tecla
Wiszniewski, Ludovic
Chanson, Marc
Published in Methods in Molecular Biology. 2011, vol. 742, p. 173-85
Abstract One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.
Keywords Animals — Cell Communication/physiology — Cell Culture Techniques — Cell Differentiation/physiology — Epithelial Cells/cytology/metabolism — Humans — Immunohistochemistry — Interleukin-8/analysis/biosynthesis — Mice — Models, Biological — Mucins/analysis/biosynthesis — Research Design — Respiratory Mucosa/cytology/metabolism — Wound Healing/physiology
Identifiers
PMID: 21547732
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Article (Published version) (683 Kb) - public document Free access
Structures
Faculté de médecine / Section de médecine clinique / Département de pédiatrie
Research group Mucoviscidose et jonctions GAP (229)
Citation
(ISO format) 		CRESPIN, Sophie et al. Approaches to study differentiation and repair of human airway epithelial cells. In: Methods in Molecular Biology, 2011, vol. 742, p. 173-85. https://archive-ouverte.unige.ch/unige:21534

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Deposited on : 2012-06-07

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