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Scientific article
Open access
English

Approaches to study differentiation and repair of human airway epithelial cells

Published inMethods in molecular biology, vol. 742, p. 173-185
Publication date2011
Abstract

One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.

Keywords
  • Animals
  • Cell Communication/physiology
  • Cell Culture Techniques
  • Cell Differentiation/physiology
  • Epithelial Cells/cytology/metabolism
  • Humans
  • Immunohistochemistry
  • Interleukin-8/analysis/biosynthesis
  • Mice
  • Models, Biological
  • Mucins/analysis/biosynthesis
  • Research Design
  • Respiratory Mucosa/cytology/metabolism
  • Wound Healing/physiology
Citation (ISO format)
CRESPIN, Sophie et al. Approaches to study differentiation and repair of human airway epithelial cells. In: Methods in molecular biology, 2011, vol. 742, p. 173–185. doi: 10.1007/978-1-61779-120-8_10
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Article (Published version)
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Identifiers
ISSN of the journal1064-3745
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Technical informations

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