Approaches to study differentiation and repair of human airway epithelial cells

Crespin, Sophie; Bacchetta, Marc; Huang, Song; Dudez, Tecla; Wiszniewski, Ludovic; Chanson, Marc

doi:10.1007/978-1-61779-120-8_10

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Title		Approaches to study differentiation and repair of human airway epithelial cells
Authors		Crespin, Sophie Bacchetta, Marc Huang, Song Dudez, Tecla Wiszniewski, Ludovic Chanson, Marc
Published in		Methods in molecular biology. 2011, vol. 742, p. 173-85
Abstract		One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.
Keywords		Animals — Cell Communication/physiology — Cell Culture Techniques — Cell Differentiation/physiology — Epithelial Cells/cytology/metabolism — Humans — Immunohistochemistry — Interleukin-8/analysis/biosynthesis — Mice — Models, Biological — Mucins/analysis/biosynthesis — Research Design — Respiratory Mucosa/cytology/metabolism — Wound Healing/physiology
Identifiers		DOI: 10.1007/978-1-61779-120-8_10 PMID: 21547732
Full text		Article (Published version) (683 Kb) - Free access
Structures		Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Research group		Mucoviscidose et jonctions GAP (229)
Citation (ISO format)		CRESPIN, Sophie et al. Approaches to study differentiation and repair of human airway epithelial cells. In: Methods in molecular biology, 2011, vol. 742, p. 173-85. doi: 10.1007/978-1-61779-120-8_10 https://archive-ouverte.unige.ch/unige:21534