An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

Holm, Frida; Ström, Susanne; Inzunza, José; Baker, Duncan; Strömberg, Anne-Marie; Rozell, Björn; Feki, Anis; Bergström, Rosita; Hovatta, Outi

doi:10.1093/humrep/deq040

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Title		An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells
Authors		Holm, Frida Ström, Susanne Inzunza, José Baker, Duncan Strömberg, Anne-Marie Rozell, Björn Feki, Anis Bergström, Rosita Hovatta, Outi show all authors [1 - 9]
Published in		Human Reproduction. 2010, vol. 25, no. 5, p. 1271-9
Abstract		Both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) bear a great potential in regenerative medicine. In addition to optimized clinical grade culture conditions, efficient clinical grade cryopreservation methods for these cells are needed. Obtaining good survival after thawing has been problematic.
Keywords		Base Sequence — Cell Differentiation — Cell Proliferation — Cell Survival — Colony-Forming Units Assay — Cryopreservation/methods — Cryoprotective Agents — Culture Media, Serum-Free — DNA Primers/genetics — Dimethyl Sulfoxide — Embryonic Stem Cells/cytology/metabolism — Gene Expression — Glucose — Homeodomain Proteins/genetics/metabolism — Humans — Immunohistochemistry — Karyotyping — Octamer Transcription Factor-3/genetics/metabolism — Pluripotent Stem Cells/cytology/metabolism — Polymerase Chain Reaction — Polymers — RNA, Messenger/genetics/metabolism — Teratoma/etiology
Identifiers		DOI: 10.1093/humrep/deq040 PMID: 20208061
Full text		Article (Published version) (641 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Citation (ISO format)		HOLM, Frida et al. An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells. In: Human Reproduction, 2010, vol. 25, n° 5, p. 1271-9. https://archive-ouverte.unige.ch/unige:21438