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Title

An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

Authors
Holm, Frida
Ström, Susanne
Inzunza, José
Baker, Duncan
Strömberg, Anne-Marie
Rozell, Björn
Bergström, Rosita
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Published in Human Reproduction. 2010, vol. 25, no. 5, p. 1271-9
Abstract Both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) bear a great potential in regenerative medicine. In addition to optimized clinical grade culture conditions, efficient clinical grade cryopreservation methods for these cells are needed. Obtaining good survival after thawing has been problematic.
Keywords Base SequenceCell DifferentiationCell ProliferationCell SurvivalColony-Forming Units AssayCryopreservation/methodsCryoprotective AgentsCulture Media, Serum-FreeDNA Primers/geneticsDimethyl SulfoxideEmbryonic Stem Cells/cytology/metabolismGene ExpressionGlucoseHomeodomain Proteins/genetics/metabolismHumansImmunohistochemistryKaryotypingOctamer Transcription Factor-3/genetics/metabolismPluripotent Stem Cells/cytology/metabolismPolymerase Chain ReactionPolymersRNA, Messenger/genetics/metabolismTeratoma/etiology
Identifiers
PMID: 20208061
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HOLM, Frida et al. An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells. In: Human Reproduction, 2010, vol. 25, n° 5, p. 1271-9. https://archive-ouverte.unige.ch/unige:21438

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Deposited on : 2012-05-25

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