An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

Holm, Frida; Ström, Susanne; Inzunza, José; Baker, Duncan; Strömberg, Anne-Marie; Rozell, Björn; Feki, Anis; Bergström, Rosita; Hovatta, Outi

doi:10.1093/humrep/deq040

Professional article

English

An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

ContributorsHolm, Frida; Ström, Susanne; Inzunza, José; Baker, Duncan; Strömberg, Anne-Marie; Rozell, Björn; Feki, Anis; Bergström, Rosita; Hovatta, Outi

Published inHuman reproduction, vol. 25, no. 5, p. 1271-1279

Publication date2010

Abstract

Both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) bear a great potential in regenerative medicine. In addition to optimized clinical grade culture conditions, efficient clinical grade cryopreservation methods for these cells are needed. Obtaining good survival after thawing has been problematic.

Keywords

Base Sequence
Cell Differentiation
Cell Proliferation
Cell Survival
Colony-Forming Units Assay
Cryopreservation/methods
Cryoprotective Agents
Culture Media, Serum-Free
DNA Primers/genetics
Dimethyl Sulfoxide
Embryonic Stem Cells/cytology/metabolism
Gene Expression
Glucose
Homeodomain Proteins/genetics/metabolism
Humans
Immunohistochemistry
Karyotyping
Octamer Transcription Factor-3/genetics/metabolism
Pluripotent Stem Cells/cytology/metabolism
Polymerase Chain Reaction
Polymers
RNA, Messenger/genetics/metabolism
Teratoma/etiology

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Citation (ISO format)

HOLM, Frida et al. An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells. In: Human reproduction, 2010, vol. 25, n° 5, p. 1271–1279. doi: 10.1093/humrep/deq040

Article (Published version)

Identifiers

PID : unige:21438
DOI : 10.1093/humrep/deq040
PMID : 20208061

Journal ISSN0268-1161

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An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

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