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An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

Published inHuman reproduction, vol. 25, no. 5, p. 1271-1279
Publication date2010
Abstract

Both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) bear a great potential in regenerative medicine. In addition to optimized clinical grade culture conditions, efficient clinical grade cryopreservation methods for these cells are needed. Obtaining good survival after thawing has been problematic.

Keywords
  • Base Sequence
  • Cell Differentiation
  • Cell Proliferation
  • Cell Survival
  • Colony-Forming Units Assay
  • Cryopreservation/methods
  • Cryoprotective Agents
  • Culture Media, Serum-Free
  • DNA Primers/genetics
  • Dimethyl Sulfoxide
  • Embryonic Stem Cells/cytology/metabolism
  • Gene Expression
  • Glucose
  • Homeodomain Proteins/genetics/metabolism
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Octamer Transcription Factor-3/genetics/metabolism
  • Pluripotent Stem Cells/cytology/metabolism
  • Polymerase Chain Reaction
  • Polymers
  • RNA, Messenger/genetics/metabolism
  • Teratoma/etiology
Citation (ISO format)
HOLM, Frida et al. An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells. In: Human reproduction, 2010, vol. 25, n° 5, p. 1271–1279. doi: 10.1093/humrep/deq040
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Article (Published version)
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Identifiers
ISSN of the journal0268-1161
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Creation05/25/2012 10:50:00 AM
First validation05/25/2012 10:50:00 AM
Update time03/14/2023 5:36:43 PM
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