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New class of gene-termini-associated human RNAs suggests a novel RNA copying mechanism

Kapranov, Philipp
Ozsolak, Fatih
Kim, Sang Woo
Foissac, Sylvain
Lipson, Doron
Hart, Chris
Roels, Steve
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Published in Nature. 2010, vol. 466, no. 7306, p. 642-646
Abstract Small (<200 nucleotide) RNA (sRNA) profiling of human cells using various technologies demonstrates unexpected complexity of sRNAs with hundreds of thousands of sRNA species present. Genetic and in vitro studies show that these RNAs are not merely degradation products of longer transcripts but could indeed have a function. Furthermore, profiling of RNAs, including the sRNAs, can reveal not only novel transcripts, but also make clear predictions about the existence and properties of novel biochemical pathways operating in a cell. For example, sRNA profiling in human cells indicated the existence of an unknown capping mechanism operating on cleaved RNA, a biochemical component of which was later identified. Here we show that human cells contain a novel type of sRNA that has non-genomically encoded 5' poly(U) tails. The presence of these RNAs at the termini of genes, specifically at the very 3' ends of known mRNAs, strongly argues for the presence of a yet uncharacterized endogenous biochemical pathway in cells that can copy RNA. We show that this pathway can operate on multiple genes, with specific enrichment towards transcript-encoding components of the translational machinery. Finally, we show that genes are also flanked by sense, 3' polyadenylated sRNAs that are likely to be capped.
Keywords Base SequenceGenes/*geneticsHeLa CellsHumansModels, GeneticNucleotides/geneticsPoly A/genetics/metabolismPoly U/genetics/metabolismRNA/biosynthesis/*classification/genetics/*metabolismRNA, Antisense/classification/genetics/metabolismTemplates, Genetic
PMID: 20671709
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Research group Pathologie Moléculaire de la Trisomie 21 et le Génome Humain (248)
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KAPRANOV, Philipp et al. New class of gene-termini-associated human RNAs suggests a novel RNA copying mechanism. In: Nature, 2010, vol. 466, n° 7306, p. 642-646. https://archive-ouverte.unige.ch/unige:20639

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Deposited on : 2012-05-22

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