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Serial chimerism analyses indicate that mixed haemopoietic chimerism influences the probability of graft rejection and disease recurrence following allogeneic stem cell transplantation (SCT) for severe aplastic anaemia (SAA): indication for routine assessment of chimerism post SCT for SAA

Authors
Lawler, Mark
McCann, Shaun R.
Marsh, Judith C. W.
Ljungman, Per
Hows, Jill
Vandenberghe, Elisabeth
O'Riordan, Joan
Locasciulli, Anna
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Published in British Journal of Haematology. 2009, vol. 144, no. 6, p. 933-945
Abstract Ninety-one patients were studied serially for chimeric status following allogeneic stem cell transplantation (SCT) for severe aplastic anaemia (SAA) or Fanconi Anaemia (FA). Short tandem repeat polymerase chain reaction (STR-PCR) was used to stratify patients into five groups: (A) complete donor chimeras (n = 39), (B) transient mixed chimeras (n = 15) (C) stable mixed chimeras (n = 18), (D) progressive mixed chimeras (n = 14) (E) recipient chimeras with early graft rejection (n = 5). As serial sampling was not possible in Group E, serial chimerism results for 86 patients were available for analysis. The following factors were analysed for association with chimeric status: age, sex match, donor type, aetiology of aplasia, source of stem cells, number of cells engrafted, conditioning regimen, graft-versus-host disease (GvHD) prophylaxis, occurrence of acute and chronic GvHD and survival. Progressive mixed chimeras (PMCs) were at high risk of late graft rejection (n = 10, P < 0.0001). Seven of these patients lost their graft during withdrawal of immunosuppressive therapy. STR-PCR indicated an inverse correlation between detection of recipient cells post-SCT and occurrence of acute GvHD (P = 0.008). PMC was a bad prognostic indicator of survival (P = 0.003). Monitoring of chimeric status during cyclosporin withdrawal may facilitate therapeutic intervention to prevent late graft rejection in patients transplanted for SAA.
Keywords AdolescentAdultAnemia, Aplastic/genetics/mortality/*therapyChildChild, PreschoolChimerismCyclosporine/therapeutic useFanconi Anemia/genetics/mortality/therapyFemaleFollow-Up Studies*Graft RejectionHumansImmunosuppressive Agents/therapeutic useMaleMiddle AgedPolymerase Chain Reaction/methodsPrognosisProportional Hazards ModelsRecurrenceStem Cell Transplantation/*adverse effectsSurvival RateTandem Repeat SequencesTime FactorsTransplantation ConditioningTransplantation, HomologousYoung Adult
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PMID: 19183198
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LAWLER, Mark et al. Serial chimerism analyses indicate that mixed haemopoietic chimerism influences the probability of graft rejection and disease recurrence following allogeneic stem cell transplantation (SCT) for severe aplastic anaemia (SAA): indication for routine assessment of chimerism post SCT for SAA. In: British Journal of Haematology, 2009, vol. 144, n° 6, p. 933-945. https://archive-ouverte.unige.ch/unige:19915

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Deposited on : 2012-04-23

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