UNIGE document Scientific Article
previous document  unige:19837  next document
add to browser collection

Proteomic analysis of mononuclear cells of patients with minimal-change nephrotic syndrome of childhood

Neuhaus, Thomas
Kemper, Markus J.
Published in Nephrology, dialysis, transplantation. 2009, vol. 24, no. 1, p. 149-155
Abstract Background/Aims. Recently, peripheral blood mononuclear cell transcriptome analysis has identified genes that are upregulated in relapsing minimal-change nephrotic syndrome (MCNS). In order to investigate protein expression in peripheral blood mononuclear cells (PBMC) from relapsing MCNS patients, we performed proteomic comparisons of PBMC from patients with MCNS in relapse and controls. METHODS: PBMC from a total of 20 patients were analysed. PBMC were taken from five patients with relapsing MCNS, four in remission, five patients with other glomerular diseases and six controls. Two dimensional electrophoresis was performed and proteome patterns were compared. RESULTS: Automatic heuristic clustering analysis allowed us to pool correctly the gels from the MCNS patients in the relapse and in the control groups. Using hierarchical population matching, nine spots were found to be increased in PBMC from MCNS patients in relapse. Four spots were identified by mass spectrometry. Three of the four proteins identified (L-plastin, alpha-tropomyosin and annexin III) were cytoskeletal-associated proteins. Using western blot and immunochemistry, L-plastin and alpha-tropomyosin 3 concentrations were found to be enhanced in PBMC from MCNS patients in relapse. Conclusions. These data indicate that a specific proteomic profile characterizes PBMC from MCNS patients in relapse. Proteins involved in PBMC cytoskeletal rearrangement are increased in relapsing MCNS. We hypothesize that T-cell cytoskeletal rearrangement may play a role in the pathogenesis of MCNS by altering the expression of cell surface receptors and by modifying the interaction of these cells with glomerular cells.
Keywords AdolescentAnnexin A3/bloodBlood Protein ElectrophoresisCase-Control StudiesChildChild, PreschoolElectrophoresis, Gel, Two-DimensionalFemaleHumansLeukocytes, Mononuclear/*metabolismMaleMicrofilament Proteins/bloodNephrosis, Lipoid/*bloodProteomicsRecurrenceT-Lymphocytes/metabolismTropomyosin/blood
PMID: 18697798
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://ndt.oxfordjournals.org/content/24/1/149.full.pdf
Research group Pathogénèse du syndrome néphrotique idiopathique de l'enfant (180)
(ISO format)
GONZALEZ, Elsa et al. Proteomic analysis of mononuclear cells of patients with minimal-change nephrotic syndrome of childhood. In: Nephrology, dialysis, transplantation, 2009, vol. 24, n° 1, p. 149-155. doi: 10.1093/ndt/gfn459 https://archive-ouverte.unige.ch/unige:19837

485 hits

0 download


Deposited on : 2012-04-23

Export document
Format :
Citation style :