UNIGE document Scientific Article
previous document  unige:19825  next document
add to browser collection

Respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults

Aubert, J-D
Thomas, Y.
show hidden authors show all authors [1 - 9]
Published in Thorax. 2009, vol. 64, no. 5, p. 399-404
Abstract BACKGROUND: The epidemiology of respiratory viruses and their potential clinical impact when recovered in lower respiratory specimens has not been established in the hospital setting. A study was performed to investigate the association between positive viral detection and respiratory infection in an at-risk population. METHODS: 299 adult patients who underwent bronchoalveolar lavage (BAL) procedures were enrolled in a hospital-based prospective cohort study. Descriptive epidemiology is presented of 17 different respiratory viruses detected by reverse transcription-polymerase chain reaction assays in BAL fluid specimens. Multivariate analysis was conducted to identify the clinical characteristics independently associated with the presence of virus. RESULTS: Of 522 BAL fluid specimens analysed, 81% were collected in adult transplant recipients or other immunocompromised patients. Overall, PCR assays identified viral nucleic acid in 91 BAL fluid samples (17.4%). Similar rates of virus-positive BAL fluid were found in the different subpopulations studied (p = 0.113). Coronaviruses were the most frequent (32.3%), followed by rhinovirus (22.6%), parainfluenza (19.5%), influenza (9.7%), respiratory synctial virus (8.6%), human metapneumovirus (4.2%) and bocavirus (3.1%). Multivariate analysis using mixed models showed that respiratory viral infections were associated with a lack of antibiotic treatment response (OR 2.2, 95% CI 1.2 to 4.1) and the absence of radiological infiltrate (OR 0.3, 95% CI 0.2 to 0.8). In lung transplant recipients in whom a respiratory infection was suspected, the respiratory viral detection rate was 24.4% compared with 13.8% overall in other patients (p = 0.02). CONCLUSIONS: In this cohort of hospitalised adults, respiratory viruses detected in BAL fluid specimens were associated with respiratory symptoms, absence of radiological infiltrates and a poor response to antibiotic therapy.
Keywords Bronchoalveolar Lavage Fluid/*virologyCohort StudiesCross Infection/*virologyFemaleHospitalizationHumansLung TransplantationMaleMiddle AgedOpportunistic Infections/*diagnosis/virologyRespiratory Tract Infections/diagnosis/*virologyReverse Transcriptase Polymerase Chain ReactionSeasonsVirus Diseases/diagnosis/*virologyViruses/*isolation & purification
PMID: 19174425
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://thorax.bmj.com/content/64/5/399.full.pdf
Research groups Pneumologie Clinique (576)
Groupe Laurent Kaiser (virologie clinique) (668)
L'épithélium respiratoire (852)
(ISO format)
GARBINO, Jorge et al. Respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults. In: Thorax, 2009, vol. 64, n° 5, p. 399-404. doi: 10.1136/thx.2008.105155 https://archive-ouverte.unige.ch/unige:19825

410 hits

0 download


Deposited on : 2012-04-23

Export document
Format :
Citation style :