Scientific article

Postsynaptic density protein PSD-95 expression in Alzheimer's disease and okadaic acid induced neuritic retraction

Published inNeurobiology of disease, vol. 30, no. 3, p. 408-419
Publication date2008

In order to understand how plasticity is related to neurodegeneration, we studied synaptic proteins with quantitative immunohistochemistry in the entorhinal cortex from Alzheimer patients and age-matched controls. We observed a significant decrease in presynaptic synaptophysin and an increase in postsynaptic density protein PSD-95, positively correlated with beta amyloid and phosphorylated Tau proteins in Alzheimer cases. Furthermore, Alzheimer-like neuritic retraction was generated in okadaic acid (OA) treated SH-SY5Y neuroblastoma cells with no decrease in PSD-95 expression. However, in a SH-SY5Y clone with decreased expression of transcription regulator LMO4 (as observed in Alzheimer's disease) and increased neuritic length, PSD-95 expression was enhanced but did not change with OA treatment. Therefore, increased PSD-95 immunoreactivity in the entorhinal cortex might result from compensatory mechanisms, as in the SH-SY5Y clone, whereas increased Alzheimer-like Tau phosphorylation is not related to PSD-95 expression, as suggested by the OA-treated cell models.

  • Adult
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease/genetics/*metabolism/pathology
  • Amyloid beta-Peptides/metabolism
  • Cell Line, Tumor
  • Cell Survival/drug effects/physiology
  • Female
  • Gene Expression Regulation/drug effects/*physiology
  • Humans
  • Intracellular Signaling Peptides and Proteins/genetics
  • Male
  • Membrane Proteins/*biosynthesis/genetics
  • Middle Aged
  • Neurites/drug effects/pathology/*physiology
  • Okadaic Acid/*toxicity
  • Phosphorylation
  • Up-Regulation/genetics
  • Tau Proteins/metabolism
Citation (ISO format)
LEUBA, Genevieve et al. Postsynaptic density protein PSD-95 expression in Alzheimer’s disease and okadaic acid induced neuritic retraction. In: Neurobiology of disease, 2008, vol. 30, n° 3, p. 408–419. doi: 10.1016/j.nbd.2008.02.012
Updates (1)
ISSN of the journal0969-9961

Technical informations

Creation03/27/2012 9:23:53 AM
First validation03/27/2012 9:23:53 AM
Update time03/14/2023 5:21:11 PM
Status update03/14/2023 5:21:10 PM
Last indexation08/28/2023 9:19:48 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack