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Postsynaptic density protein PSD-95 expression in Alzheimer's disease and okadaic acid induced neuritic retraction
|Published in||Neurobiology of Disease. 2008, vol. 30, no. 3, p. 408-419|
|Abstract||In order to understand how plasticity is related to neurodegeneration, we studied synaptic proteins with quantitative immunohistochemistry in the entorhinal cortex from Alzheimer patients and age-matched controls. We observed a significant decrease in presynaptic synaptophysin and an increase in postsynaptic density protein PSD-95, positively correlated with beta amyloid and phosphorylated Tau proteins in Alzheimer cases. Furthermore, Alzheimer-like neuritic retraction was generated in okadaic acid (OA) treated SH-SY5Y neuroblastoma cells with no decrease in PSD-95 expression. However, in a SH-SY5Y clone with decreased expression of transcription regulator LMO4 (as observed in Alzheimer's disease) and increased neuritic length, PSD-95 expression was enhanced but did not change with OA treatment. Therefore, increased PSD-95 immunoreactivity in the entorhinal cortex might result from compensatory mechanisms, as in the SH-SY5Y clone, whereas increased Alzheimer-like Tau phosphorylation is not related to PSD-95 expression, as suggested by the OA-treated cell models.|
|Keywords||Adult — Aged — Aged, 80 and over — Alzheimer Disease/genetics/*metabolism/pathology — Amyloid beta-Peptides/metabolism — Cell Line, Tumor — Cell Survival/drug effects/physiology — Female — Gene Expression Regulation/drug effects/*physiology — Humans — Intracellular Signaling Peptides and Proteins/genetics — Male — Membrane Proteins/*biosynthesis/genetics — Middle Aged — Neurites/drug effects/pathology/*physiology — Okadaic Acid/*toxicity — Phosphorylation — Up-Regulation/genetics — Tau Proteins/metabolism|
|Research group||Groupe Bouras Constantin (neuropsychiatrie) (1)|
|LEUBA, Genevieve et al. Postsynaptic density protein PSD-95 expression in Alzheimer's disease and okadaic acid induced neuritic retraction. In: Neurobiology of Disease, 2008, vol. 30, n° 3, p. 408-419. doi: 10.1016/j.nbd.2008.02.012 https://archive-ouverte.unige.ch/unige:19185|