Contributions to Bax insertion and oligomerization of lipids of the mitochondrial outer membrane

Lucken-Ardjomande, Safa; Montessuit, Sylvie; Martinou, Jean-Claude

doi:10.1038/cdd.2008.9

Scientific article

English

Contributions to Bax insertion and oligomerization of lipids of the mitochondrial outer membrane

Contributors

Lucken-Ardjomande, Safa; Montessuit, Sylvie; Martinou, Jean-Claude

Published in

Cell death and differentiation, vol. 15, p. 929-937

Publication date

2008

Abstract

Under many apoptotic conditions, Bax undergoes conformational rearrangements, leading to its insertion in the mitochondrial outer membrane as a transmembrane oligomer. At the same time, mitochondria undergo fragmentation and activated Bax was reported to localize to fission sites. We studied how lipid composition and membrane curvature regulate Bax activation. When isolated mitochondria were incubated with phospholipase A(2), which led to phosphatidylethanolamine and cardiolipin hydrolysis, tBid and Bax insertion were hindered. We thus studied in liposomes how phosphatidylethanolamine, cardiolipin, and its hydrolysis products affect Bax activation. Whereas phosphatidylethanolamine, a lipid with negative curvature, did not affect Bax insertion, it inhibited Bax oligomerization. Conversely, Bax insertion required cardiolipin, and was not blocked by cardiolipin hydrolysis products. These experiments support a direct role for cardiolipin in the recruitment and activation of Bax. To examine if the increase in membrane curvature that accompanies mitochondrial fission participates in Bax activation, we studied how liposome size affects the process, and observed that it was inhibited in small liposomes (

UNIGE affiliation entities

Faculté des sciences / Section de biologie / Département de biologie cellulaire

Citation (ISO format)

LUCKEN-ARDJOMANDE, Safa, MONTESSUIT, Sylvie, MARTINOU, Jean-Claude. Contributions to Bax insertion and oligomerization of lipids of the mitochondrial outer membrane. In: Cell death and differentiation, 2008, vol. 15, p. 929–937. doi: 10.1038/cdd.2008.9

Article (Published version)

Identifiers

PID : unige:19
DOI : 10.1038/cdd.2008.9
PMID : 18259190

Journal ISSN

1350-9047

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Creation

29/10/2008 11:37:32

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29/10/2008 11:37:32

Update

14/03/2023 14:56:29

Status update

14/03/2023 14:56:29

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29/10/2024 10:47:10

Contributions to Bax insertion and oligomerization of lipids of the mitochondrial outer membrane

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

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Creation

First validation

Update

Status update

Last indexation