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Title

Normalization of stratum corneum barrier function and transepidermal water loss in vivo
Authors
Kalia, Yogeshvar
Alberti, Ingo
Sekkat, Nabila
Curdy, Catherine Anne-Marie
Naik, Aarti
Guy, Richard H.
Published in Pharmaceutical Research. 2000, vol. 17, no. 9, p. 1148-50
Abstract PURPOSE : To understand and account for inter-individual variations in percutaneous drug penetration and transepidermal water loss (TEWL). METHODS: TEWL is the standard measure of stratum corneum (SC) barrier function in vivo. The use of serial tape-stripping enables SC barrier efficiency to be assessed as a function of position. Previous studies have shown that TEWL increases as the barrier is removed by progressive tape-stripping. Although complete removal of the SC results in TEWL increasing to 80-100 g/m2h, inter-individual (and inter-regional) differences in SC thickness mean that different amounts of tissue have to be removed before these TEWL levels are attained. RESULTS: We show that normalization of the amount of SC removed with respect to the total Sc thickness effectively eliminates the inter-individual differences perceived in the raw data (i.e., TEWL as a function of tape-stripping number or as a function of cumulative SC mass removed). CONCLUSIONS: The results of this study demonstrate clearly that the objective evaluation of topical drug bioavailability (or of topical bioequivalence between different formulations) using tape-stripping methodology, as has been recently advocated by the U.S. Food & Drug Administration, must involve a quantitative determination of the amount of SC removed. In this way, the significant inter-individual differences typically observed in the penetration measurements may be decreased.
Keywords Adult — Diffusion — Epidermis/physiology — Female — Humans — Male — Water Loss, Insensible/physiology
Identifiers
PMID: 11087050
Full text
Article (Published version) (100 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		KALIA, Yogeshvar et al. Normalization of stratum corneum barrier function and transepidermal water loss in vivo. In: Pharmaceutical Research, 2000, vol. 17, n° 9, p. 1148-50. https://archive-ouverte.unige.ch/unige:18738

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Deposited on : 2012-03-06

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