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Titles listNormalization of stratum corneum barrier function and transepidermal water loss in vivo
Titles listNormalization of stratum corneum barrier function and transepidermal water loss in vivo
Scientific Article
unige:18738 
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Normalization of stratum corneum barrier function and transepidermal water loss in vivo |
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| Authors | ||
| Published in | Pharmaceutical Research. 2000, vol. 17, no. 9, p. 1148-50 | |
| Abstract | PURPOSE : To understand and account for inter-individual variations in percutaneous drug penetration and transepidermal water loss (TEWL). METHODS: TEWL is the standard measure of stratum corneum (SC) barrier function in vivo. The use of serial tape-stripping enables SC barrier efficiency to be assessed as a function of position. Previous studies have shown that TEWL increases as the barrier is removed by progressive tape-stripping. Although complete removal of the SC results in TEWL increasing to 80-100 g/m2h, inter-individual (and inter-regional) differences in SC thickness mean that different amounts of tissue have to be removed before these TEWL levels are attained. RESULTS: We show that normalization of the amount of SC removed with respect to the total Sc thickness effectively eliminates the inter-individual differences perceived in the raw data (i.e., TEWL as a function of tape-stripping number or as a function of cumulative SC mass removed). CONCLUSIONS: The results of this study demonstrate clearly that the objective evaluation of topical drug bioavailability (or of topical bioequivalence between different formulations) using tape-stripping methodology, as has been recently advocated by the U.S. Food & Drug Administration, must involve a quantitative determination of the amount of SC removed. In this way, the significant inter-individual differences typically observed in the penetration measurements may be decreased. | |
| Keywords | Adult — Diffusion — Epidermis/physiology — Female — Humans — Male — Water Loss, Insensible/physiology | |
| Identifiers | PMID: 11087050 | |
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| Citation (ISO format) | KALIA, Yogeshvar et al. Normalization of stratum corneum barrier function and transepidermal water loss in vivo. In: Pharmaceutical Research, 2000, vol. 17, n° 9, p. 1148-50. doi: 10.1023/A:1026474200575 https://archive-ouverte.unige.ch/unige:18738 |
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