UNIGE document Scientific Article
previous document  unige:18253  next document
add to browser collection
Title

Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation

Authors
Davis, Nicole J.
Published in FEMS Microbiology Letters. 2011, vol. 319, no. 2, p. 146-52
Abstract In the asymmetric predivisional cell of Caulobacter crescentus, TipF and TipN mark the cellular pole for future flagellar development. TipF is essential for motility and contains a cyclic-di-GMP phosphodiesterase-like (EAL) domain that is necessary for proper function. TipN is localized to the flagellar pole before TipF and is essential for the proper placement of the flagellum in C. crescentus. Using β-galactosidase promoter-probe assays and quantitative chromatin immunoprecipitation, we investigated the influence of the C. crescentus flagellar assembly regulator TipF on flagellar gene transcription. We compared the transcriptional activity of class II-fliF-lacZ, class III-flgE-lacZ, and class IV-fljL-lacZ fusions in a ΔtipF mutant with that of other flagellar mutants and the wild-type strain. We subsequently verified the in vivo occupancy of the fliF, flgE, and fljL flagellar promoters by the flagellar regulators CtrA, FlbD, and FliX in addition to RNA polymerase. We deduce that TipF contributes to proper expression of flagellar genes in C. crescentus by acting both within and outside of the canonical flagellar gene expression hierarchy.
Keywords Bacterial Proteins/chemistry/genetics/metabolismBinding SitesCaulobacter crescentus/chemistry/genetics/metabolismChromatin Immunoprecipitation/methodsDNA-Binding Proteins/chemistry/genetics/metabolismFlagella/chemistry/genetics/metabolismGene Expression Regulation, BacterialMutationPromoter Regions, GeneticTranscription, Genetic
Identifiers
PMID: 21457294
Full text
Article (Published version) (283 Kb) - public document Free access
Structures
Citation
(ISO format)
DAVIS, Nicole J., VIOLLIER, Patrick. Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation. In: FEMS Microbiology Letters, 2011, vol. 319, n° 2, p. 146-52. https://archive-ouverte.unige.ch/unige:18253

223 hits

765 downloads

Update

Deposited on : 2012-01-31

Export document
Format :
Citation style :