Scientific article
Open access

Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation

Published inFEMS microbiology letters, vol. 319, no. 2, p. 146-152
Publication date2011

In the asymmetric predivisional cell of Caulobacter crescentus, TipF and TipN mark the cellular pole for future flagellar development. TipF is essential for motility and contains a cyclic-di-GMP phosphodiesterase-like (EAL) domain that is necessary for proper function. TipN is localized to the flagellar pole before TipF and is essential for the proper placement of the flagellum in C. crescentus. Using β-galactosidase promoter-probe assays and quantitative chromatin immunoprecipitation, we investigated the influence of the C. crescentus flagellar assembly regulator TipF on flagellar gene transcription. We compared the transcriptional activity of class II-fliF-lacZ, class III-flgE-lacZ, and class IV-fljL-lacZ fusions in a ΔtipF mutant with that of other flagellar mutants and the wild-type strain. We subsequently verified the in vivo occupancy of the fliF, flgE, and fljL flagellar promoters by the flagellar regulators CtrA, FlbD, and FliX in addition to RNA polymerase. We deduce that TipF contributes to proper expression of flagellar genes in C. crescentus by acting both within and outside of the canonical flagellar gene expression hierarchy.

  • Bacterial Proteins/chemistry/genetics/metabolism
  • Binding Sites
  • Caulobacter crescentus/chemistry/genetics/metabolism
  • Chromatin Immunoprecipitation/methods
  • DNA-Binding Proteins/chemistry/genetics/metabolism
  • Flagella/chemistry/genetics/metabolism
  • Gene Expression Regulation, Bacterial
  • Mutation
  • Promoter Regions, Genetic
  • Transcription, Genetic
Citation (ISO format)
DAVIS, Nicole J., VIOLLIER, Patrick. Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation. In: FEMS microbiology letters, 2011, vol. 319, n° 2, p. 146–152. doi: 10.1111/j.1574-6968.2011.02275.x
Main files (1)
Article (Published version)
ISSN of the journal0378-1097

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