• Home
  1. Home
  2. Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation
Scientific article
OA Policy
English

Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation

Contributors

Davis, Nicole J.; Viollier, Patrick

Published in

FEMS microbiology letters, vol. 319, no. 2, p. 146-152

Publication date

2011

Abstract

In the asymmetric predivisional cell of Caulobacter crescentus, TipF and TipN mark the cellular pole for future flagellar development. TipF is essential for motility and contains a cyclic-di-GMP phosphodiesterase-like (EAL) domain that is necessary for proper function. TipN is localized to the flagellar pole before TipF and is essential for the proper placement of the flagellum in C. crescentus. Using β-galactosidase promoter-probe assays and quantitative chromatin immunoprecipitation, we investigated the influence of the C. crescentus flagellar assembly regulator TipF on flagellar gene transcription. We compared the transcriptional activity of class II-fliF-lacZ, class III-flgE-lacZ, and class IV-fljL-lacZ fusions in a ΔtipF mutant with that of other flagellar mutants and the wild-type strain. We subsequently verified the in vivo occupancy of the fliF, flgE, and fljL flagellar promoters by the flagellar regulators CtrA, FlbD, and FliX in addition to RNA polymerase. We deduce that TipF contributes to proper expression of flagellar genes in C. crescentus by acting both within and outside of the canonical flagellar gene expression hierarchy.

Keywords

  • Bacterial Proteins/chemistry/genetics/metabolism
  • Binding Sites
  • Caulobacter crescentus/chemistry/genetics/metabolism
  • Chromatin Immunoprecipitation/methods
  • DNA-Binding Proteins/chemistry/genetics/metabolism
  • Flagella/chemistry/genetics/metabolism
  • Gene Expression Regulation, Bacterial
  • Mutation
  • Promoter Regions, Genetic
  • Transcription, Genetic

UNIGE affiliation entities

Citation (ISO format)

DAVIS, Nicole J., VIOLLIER, Patrick. Probing flagellar promoter occupancy in wild-type and mutant Caulobacter crescentus by chromatin immunoprecipitation. In: FEMS microbiology letters, 2011, vol. 319, n° 2, p. 146–152. doi: 10.1111/j.1574-6968.2011.02275.x

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0378-1097
680views
965downloads

Technical informations

Creation

27/01/2012 20:53:00

First validation

27/01/2012 20:53:00

Update

14/10/2025 00:23:39

Status update

14/03/2023 17:07:41

Last indexation

02/11/2026 16:33:34
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated