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Scientific article
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English

Transgene- and locus-dependent imprinting reveals allele-specific chromosome conformations

Publication date2013-07-16
First online date2013-07
Abstract

When positioned into the integrin α-6 gene, an Hoxd9lacZ reporter transgene displayed parental imprinting in mouse embryos. While the expression from the paternal allele was comparable with patterns seen for the same transgene when present at the neighboring HoxD locus, almost no signal was scored at this integration site when the transgene was inherited from the mother, although the Itga6 locus itself is not imprinted. The transgene exhibited maternal allele-specific DNA hypermethylation acquired during oogenesis, and its expression silencing was reversible on passage through the male germ line. Histone modifications also corresponded to profiles described at known imprinted loci. Chromosome conformation analyses revealed distinct chromatin microarchitectures, with a more compact structure characterizing the maternally inherited repressed allele. Such genetic analyses of well-characterized transgene insertions associated with a de novo-induced parental imprint may help us understand the molecular determinants of imprinting.

eng
Keywords
  • Hox genes
  • Architecture
  • Enhancer
  • Epigenetic
Citation (ISO format)
LONFAT, Nicolas et al. Transgene- and locus-dependent imprinting reveals allele-specific chromosome conformations. In: Proceedings of the National Academy of Sciences of the United States of America, 2013, vol. 110, n° 29, p. 11946–11951. doi: 10.1073/pnas.1310704110
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ISSN of the journal0027-8424
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