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Scientific article
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English

Nanoscale spatial organization of the HoxD gene cluster in distinct transcriptional states

Publication date2015-10-26
First online date2015-10-26
Abstract

Ultrastructural chromatin dynamics may play a key role in regulating transcriptional activation. Here we have used super-resolution microscopy to study the folding mechanics of the HoxD cluster, as assayed by following the elongation of chromatin in single cells with different status of Hox gene activation. We observed that the spatial separation of Hoxd genes is strongest in those tissues where they are highly expressed. We also document that the opening of chromatin precedes transcription and that the strongest elongations are observed at the location of the boundary between two major topologically associating domains (TADs). These results shed light on how spatial compartmentalization is achieved, likely to accompany efficient chromatin reorganization upon activation of transcriptional switches.

eng
Keywords
  • DNA FISH
  • HoxD
  • Limb development
  • Super-resolution microscopy
  • Topologically associating domains
Citation (ISO format)
FABRE, Pierre et al. Nanoscale spatial organization of the <i>HoxD</i> gene cluster in distinct transcriptional states. In: Proceedings of the National Academy of Sciences of the United States of America, 2015, vol. 112, n° 45, p. 13964–13969. doi: 10.1073/pnas.1517972112
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Article (Published version)
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ISSN of the journal0027-8424
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