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Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution

Rain, J C
Tartakoff, A M
Legrain, P
Published in RNA. 1996, vol. 2, no. 6, p. 535-50
Abstract The yeast Prp9p, Prp11p, Prp21p proteins form a multimolecular complex identified as the SF3a splicing factor in higher eukaryotes. This factor is required for the assembly of the prespliceosome. Prp21p interacts with both Prp9p and Prp11p, but the molecular basis of these interactions is unknown. Prp21p, its human homologue, and the so-called SWAP proteins share a tandemly repeated motif, the surp module. Given the evolutionary conservation and the role of SWAP proteins as splicing regulators, it has been proposed that surp motifs are essential for interactions between Prp21p and other splicing factors. In order to characterize functional domains of Prp21p and to identify potential additional functions of this protein, we isolated a series of heat-sensitive prp21 mutants. Our results indicate that prp21 heat-sensitive mutations are associated with defects in the interaction with Prp9p, but not with Prp11p. Interestingly, most heat-sensitive point mutants associate a strong splicing defect with a pre-mRNA nuclear export phenotype, as does the prp9-1 heat-sensitive mutant. Deletion analyses led to the definition of domains required for viability. These domains are responsible for the interaction with Prp9p and Prp11p and are conserved through evolution. They do not include the most conserved surp1 module, suggesting that the conservation of this motif in two families of proteins may reflect a still unknown function dispensable in yeast under standard conditions.
Keywords Amino Acid SequenceBinding SitesCell DivisionConserved SequenceEvolution, MolecularFungal Proteins/chemistry/genetics/metabolismHot TemperatureHumansMolecular Sequence DataMutagenesis, Site-DirectedPoint MutationRNA Precursors/metabolismRNA SplicingRNA-Binding ProteinsSaccharomyces cerevisiae/chemistry/geneticsSaccharomyces cerevisiae ProteinsSequence AlignmentSequence DeletionSpliceosomes/genetics/metabolism
PMID: 8718683
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RAIN, J C et al. Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. In: RNA, 1996, vol. 2, n° 6, p. 535-50. https://archive-ouverte.unige.ch/unige:17547

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Deposited on : 2011-11-25

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