Scientific article
OA Policy
English

Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli

Published inMolecular biology of the cell, vol. 30, no. 8, p. 975-991
Publication date2019-04
Abstract

Many fungal species are dimorphic, exhibiting both unicellular yeast-like and filamentous forms. Schizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus. Here, we first identify fruit extracts as natural, stress-free, starvation-independent inducers of filamentation, which we use to describe the properties of the dimorphic switch. During the yeast-to-hypha transition, the cell evolves from a bipolar to a unipolar system with 10-fold accelerated polarized growth but constant width, vacuoles segregated to the nongrowing half of the cell, and hyper-lengthening of the cell. We demonstrate unusual features of S. japonicus hyphae: these cells lack a Spitzenkörper, a vesicle distribution center at the hyphal tip, but display more rapid cytoskeleton-based transport than the yeast form, with actin cables being essential for the transition. S. japonicus hyphae also remain mononuclear and undergo complete cell divisions, which are highly asymmetric: one daughter cell inherits the vacuole, the other the growing tip. We show that these elongated cells scale their nuclear size, spindle length, and elongation rates, but display altered division size controls. This establishes S. japonicus as a unique system that switches between symmetric and asymmetric modes of growth and division.

Affiliation entities Not a UNIGE publication
Citation (ISO format)
KINNAER, Cassandre, DUDIN, Omaya, MARTIN, Sophie. Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli. In: Molecular biology of the cell, 2019, vol. 30, n° 8, p. 975–991. doi: 10.1091/mbc.E18-12-0774
Main files (1)
Article (Published version)
Identifiers
Journal ISSN1059-1524
43views
5downloads

Technical informations

Creation30/01/2024 17:58:45
First validation31/01/2024 15:12:15
Update time31/01/2024 15:12:15
Status update31/01/2024 15:12:15
Last indexation01/11/2024 07:22:40
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack