Aim

Immunisation is a global health priority, but methods of non‐pharmacological pain relief are not widely used in routine clinical practice. In this study, we set out to investigate the effects of maternal singing during the routine vaccination of infants.

Methods

We recruited 67 mother–infant pairs at Health Centres in the Aosta Region of Italy. Infants aged 2–4 months were randomly allocated to a singing intervention group or to a control group whose injections were administered following standard practice. Pre‐ and post‐immunisation pain was blindly assessed using the Modified Behavioural Pain Scale, and mother–infant proximity indexes were assigned based on muted video‐tracks.

Results

When assessed for pain, the infants in the maternal singing group were assigned significantly lower movement indexes ( p = 0.032) and marginally significantly lower cry indexes ( p = 0.076). A higher frequency of mother‐to‐infant gaze ( p < 0.005) was observed in the singing group dyads. Finally, the intervention group mothers' self‐perceived ease in singing was correlated with their previous singing experience and with lower anxiety following the vaccination procedure ( p < 0.05).

Conclusion

Maternal singing during immunisation procedures benefits both mothers and babies. The practice of singing is a biologically rooted and adaptive form of intuitive parental communication that should be encouraged, especially in at‐risk populations.