Scientific article
Open access

Metagenomics analysis of the neonatal intestinal resistome

Published inFrontiers in pediatrics, vol. 11, 1169651
Publication date2023
First online date2023-06-16

Introduction: The intestinal microbiome forms a major reservoir for antibiotic resistance genes (ARGs). Little is known about the neonatal intestinal resistome.

Objective: The objective of this study was to investigate the intestinal resistome and factors that influence the abundance of ARGs in a large cohort of neonates.

Methods: Shotgun metagenomics was used to analyse the resistome in stool samples collected at 1 week of age from 390 healthy, term-born neonates who did not receive antibiotics.

Results: Overall, 913 ARGs belonging to 27 classes were identified. The most abundant ARGs were those conferring resistance to tetracyclines, quaternary ammonium compounds, and macrolide-lincosamide-streptogramin-B. Phylogenetic composition was strongly associated with the resistome composition. Other factors that were associated with the abundance of ARGs were delivery mode, gestational age, birth weight, feeding method, and antibiotics in the last trimester of pregnancy. Sex, ethnicity, probiotic use during pregnancy, and intrapartum antibiotics had little effect on the abundance of ARGs.

Conclusion: Even in the absence of direct antibiotic exposure, the neonatal intestine harbours a high abundance and a variety of ARGs.

  • Antibiotic resistance
  • Gut
  • Infant
  • Microbiome
  • Microbiota
  • Mobilome
  • Stool
Citation (ISO format)
LEO, Stefano et al. Metagenomics analysis of the neonatal intestinal resistome. In: Frontiers in pediatrics, 2023, vol. 11, p. 1169651. doi: 10.3389/fped.2023.1169651
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ISSN of the journal2296-2360

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