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Scientific article
Open access
English

Stratifying the Presymptomatic Phase of Genetic Frontotemporal Dementia by Serum NfL and pNfH: A Longitudinal Multicentre Study

Published inAnnals of neurology, vol. 91, no. 1, p. 33-47
Publication date2022-01
First online date2021-11-07
Abstract

Objective: Although the presymptomatic stages of frontotemporal dementia (FTD) provide a unique chance to delay or even prevent neurodegeneration by early intervention, they remain poorly defined. Leveraging a large multicenter cohort of genetic FTD mutation carriers, we provide a biomarker-based stratification and biomarker cascade of the likely most treatment-relevant stage within the presymptomatic phase: the conversion stage.

Methods: We longitudinally assessed serum levels of neurofilament light (NfL) and phosphorylated neurofilament heavy (pNfH) in the Genetic FTD Initiative (GENFI) cohort (n = 444), using single-molecule array technique. Subjects comprised 91 symptomatic and 179 presymptomatic subjects with mutations in the FTD genes C9orf72, GRN, or MAPT, and 174 mutation-negative within-family controls.

Results: In a biomarker cascade, NfL increase preceded the hypothetical clinical onset by 15 years and concurred with brain atrophy onset, whereas pNfH increase started close to clinical onset. The conversion stage was marked by increased NfL, but still normal pNfH levels, while both were increased at the symptomatic stage. Intra-individual change rates were increased for NfL at the conversion stage and for pNfH at the symptomatic stage, highlighting their respective potential as stage-dependent dynamic biomarkers within the biomarker cascade. Increased NfL levels and NfL change rates allowed identification of presymptomatic subjects converting to symptomatic disease and capture of proximity-to-onset. We estimate stage-dependent sample sizes for trials aiming to decrease neurofilament levels or change rates.

Interpretation: Blood NfL and pNfH provide dynamic stage-dependent stratification and, potentially, treatment response biomarkers in presymptomatic FTD, allowing demarcation of the conversion stage. The proposed biomarker cascade might pave the way towards a biomarker-based precision medicine approach to genetic FTD.

eng
Keywords
  • Aged
  • Biomarkers / blood
  • Cohort Studies
  • Female
  • Frontotemporal Dementia / blood
  • Humans
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Neurofilament Proteins / blood
Funding
  • European Reference Network for Rare Neurological Diseases - [739510]
  • Memorabel grants from Deltaplan Dementie - [733050103]
  • Medical Research Council - [MC_UU_00024/1]
  • Medical Research Council - [MR/M008525/1]
  • Medical Research Council - [G1100464]
  • NIHR Cambridge Biomedical Research Centre - [BRC-1215-20014]
  • Medical Research Council - [SUAG/051 G101400]
  • Medical Research Council - [MR/J009482/1]
  • NIHR Rare Disease Translational Research Collaboration - [BRC149/NS/MH]
  • EU Joint Programme - Neurodegenerative Disease Research - [2019-02248]
  • Horizon 2020 research and innovation programme - [643417]
  • Memorabel grants from Deltaplan Dementie - [733050813]
  • Medical Research Council - [MR/M023664/1]
  • MRC Clinician Scientist Fellowship - [MR/M008525/1]
  • Medical Research Council - [MR/T046015/1]
  • European Commission - Solving the unsolved Rare Diseases [779257]
  • Swiss National Science Foundation - Exploring the role of gut-microbiota in multiple sclerosis pathogenesis [191077]
Citation (ISO format)
WILKE, Carlo et al. Stratifying the Presymptomatic Phase of Genetic Frontotemporal Dementia by Serum NfL and pNfH: A Longitudinal Multicentre Study. In: Annals of neurology, 2022, vol. 91, n° 1, p. 33–47. doi: 10.1002/ana.26265
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Article (Published version)
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Identifiers
ISSN of the journal0364-5134
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Technical informations

Creation11/16/2022 12:35:17 PM
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Update time09/20/2023 3:24:10 PM
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