en
Scientific article
English

Generation of Insulin-Producing Multicellular Organoids

Published inMethods in molecular biology, vol. 2592, p. 37-60
Publication date2023
First online date2022-12-13
Abstract

Clinical islet transplantation (CIT) is an established noninvasive treatment for type I diabetes (T1D) and has demonstrated improved glycemic control, preventing the occurrence of severe hypoglycemia. However, CIT has several limitations, such as the need for multiple donors, lifelong immunosuppression, and suboptimal long-term graft function. Most of the transplanted islets are lost due to inflammation, ischemic damage, and delayed revascularization.Generation of organoids have gained increasing interest in regenerative medicine in recent years. In the context of beta-cell replacement, it offers a possibility to address limitations of CIT by allowing to produce uniform organoids from single or multiple cell types facilitating revascularization and anti-inflammatory and/or immunomodulatory protection. We have previously generated multicellular insulin-secreting organoids composed of islet cells and the human amniotic epithelial cells (hAECs). These 3D insulin-secreting structures demonstrated improved viability and function both in vitro and in vivo. Here we detail a stepwise methodology to generate insulin-secreting organoids using two different methods. In addition, quality assessment in vitro tests are also described.

eng
Keywords
  • Amniotic epithelial cells
  • Diabetes
  • EndoC-BH1
  • Insulin-secreting
  • Islets
  • Multicellular
  • Organoids
  • Humans
  • Islets of Langerhans Transplantation / methods
  • Islets of Langerhans / metabolism
  • Organoids
  • Insulin-Secreting Cells / metabolism
  • Insulin / metabolism
  • Diabetes Mellitus, Type 1 / therapy
  • Diabetes Mellitus, Type 1 / metabolism
Citation (ISO format)
OLIVEIRA ALMEIDA FONSECA, Laura Do Mar et al. Generation of Insulin-Producing Multicellular Organoids. In: Methods in molecular biology, 2023, vol. 2592, p. 37–60. doi: 10.1007/978-1-0716-2807-2_3
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Article (Published version)
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Identifiers
ISSN of the journal1064-3745
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